Extraction and separation method of prodigiosin

Abstract

The invention discloses an extraction and separation method of prodigiosin. The extraction and separation method of the prodigiosin comprises the following steps: selecting a strain optimized in a laboratory; inoculating into seed medium after beveled activation, and carrying out fermentation cultivation; extracting prodigiosin, taking a fermentation liquor, centrifuging, and adding proper acidic acetone; carrying out ultrasonic extraction and rotary evaporation by using a rotary evaporator to obtain an acetone crude extract; degreasing by using a petroleum ether; carrying out a preliminary experiment before silica gelcolumn chromatography by using a thin layer chromatography method; carrying out experiment for a plurality of times by changing the ratio, so as to obtain the best solvent ratio; carrying out column chromatography; carrying out secondary chromatography separation and purification. By adopting the method, supernatant containing a little of prodigiosin is removed in a centrifuging manner; the strain is roughly extracted by using the acidic acetone; fine separation is carried out by selecting a common silica gelcolumn chromatograph; gradient elution is carried out by adopting a chloroform-ethyl acetate solvent system and a chloroform-ethyl acetate-methanol solvent system; the prodigiosin and an unknown substance are obtained in a separation manner. Thus, the separation purity is high.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, in particular to a method for extracting and separating prodigiosin. Background technique [0002] Prodigiosin is a natural red pigment mainly produced by microorganisms. It was discovered by Amako et al. in 1929 when they were studying the growth of Serratia, and it was Harashima et al. who first isolated prodigiosin in 1960. . With the in-depth study of the properties and biological activities of prodigiosin, researchers found that it has anti-bacteria, anti-malarial, anti-fungal, anti-protozoa, and the most concerned immunosuppressive activity and the ability to cause tumor cell apoptosis Therefore, as a natural pigment, prodigiosin has great application potential and broad market prospects in the fields of medicine, food, printing and dyeing, textiles, feed additives, and environmental governance. Moreover, prodigiosin, as a pigment produced by microorganisms, overcomes many disadvanta...

AI Technical Summary

Problems solved by technology

The purpose of separation and purification of secondary metabolites of microorganisms is to obtain high-purity products from the fermentation broth that meet the quality requirements of food and medicine. However, due to the many and complex components in the fermentation broth, the cost of separation and purification is often high, accounting The proportion of the total cost of the product is very large, and the cost of separation and purification accounts for about 40-70% of different products

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