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Anabaena capable of efficiently degrading polychlorinated biphenyl and application thereof

A PCB and Anabaena technology, applied in the direction of single-cell algae, microorganism-based methods, microorganisms, etc., to achieve the effect of important application prospects

Active Publication Date: 2014-04-23
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although scholars at home and abroad have conducted research on the degradation of PCBs by microorganisms, the field of dechlorinating functional algae to degrade PCBs is still in a vacuum in my country, and there are no relevant literature and experimental reports.

Method used

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  • Anabaena capable of efficiently degrading polychlorinated biphenyl and application thereof
  • Anabaena capable of efficiently degrading polychlorinated biphenyl and application thereof
  • Anabaena capable of efficiently degrading polychlorinated biphenyl and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Separation and screening of algal species

[0043] Soil sample: collected from a PCB-contaminated rice field in Taizhou City, Zhejiang Province;

[0044] Enrichment and separation of algal species: screening with liquid medium containing 10ppm PCBs;

[0045] The medium of the present invention is: BG11 inorganic salt chlorine-free liquid medium (1000mL), the composition is shown in Table 2;

[0046]The composition of BG11 inorganic salt medium is shown in Table 1 (the solvent is water):

[0047] Table 1

[0048]

[0049] A 5 The composition of the solution is shown in Table 2 (the solvent is water):

[0050] Table 2

[0051]

[0052] The composition of BG11 inorganic salt chlorine-free medium is shown in Table 3 (the solvent is water):

[0053] table 3

[0054]

[0055] Molecular identification of algal species:

[0056] The 16SrDNA sequence of the algal species was sequenced, and the sequence shown as SEQ ID NO.1 was obtained, and the BLAST of the ...

Embodiment 2

[0062] Example 2: Anabaena species AnabaenaPD-3 biodegrades 2mg / L of PCB287 days exposure experiment

[0063] First, measure the OD value of the algae liquid (the culture medium is BG11 inorganic salt chlorine-free liquid medium, the same below) at a wavelength of 680nm with the dechlorination functional algae species PD-3 (CCTCCNo: M2013398), and adjust the OD value to 0.38. Keep the algae in logarithmic growth phase. Then add 20mL of algae solution to a clean 50mL Erlenmeyer flask, and add 400μL of PCB28 (concentration: 100mg / L) to adjust the final concentration of PCB28 to 2mg / L. Seal the Erlenmeyer flask with a sterile filter. Finally, the light intensity of the artificial climate incubator was adjusted to 1003lux respectively, the culture condition was 25°C, and the light-dark ratio was 12:12h (that is, 12h of light and 12h of darkness alternately). After 7 days of exposure culture, it was measured by mercury thiocyanate ferric photometry The concentration of chloride i...

Embodiment 3

[0064] Example 3: Anabaena species AnabaenaPD-3 biodegrades 2mg / L of PCB297 days exposure experiment

[0065] First, measure the OD value of the algae liquid with the dechlorination functional algae PD-3 at a wavelength of 680nm, and adjust the OD value to 0.38, so that the algae is in the logarithmic growth phase. Then, 20 mL of algae solution was added to a clean 50 mL Erlenmeyer flask, and 400 μL of PCB29 (concentration of 100 mg / L) was added to adjust the final concentration of PCB29 to 2 mg / L. Seal the Erlenmeyer flask with a sterile filter. Finally, the light intensity of the artificial climate incubator was adjusted to 1003 lux, the culture condition was 25°C, and the light-dark ratio was 12:12h. After 7 days of exposure culture, the chloride ion concentration was measured by mercury thiocyanate ferric photometry, and its degradation effect was studied. The degradation efficiency is up to 54%, see the degradation results figure 2 .

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Abstract

The invention provides anabaena sp. PD-3 capable of efficiently degrading polychlorinated biphenyl (PCB), and application thereof to microbiological degradation of polychlorinated biphenyl. The anabaena sp. PD-3 is collected in the China Center For Type Culture Collection, with an address of Wuhan universality, Wuhan, China, a postal code of 430072, a collection date of September 8, 2013, and a collection number of CCTCC No.M2013398. The invention provides anabaena PD-3 capable of efficiently degrading polychlorinated biphenyl. The anabaena is capable of effectively degrading PCB28, 29, 30 and 31; after the exposure for a week, the degradation efficiency of the anabaena to PCB28 exceeds 60 percent, the dechlorinating percents of the anabaena to PCB44 and PCB61 are respectively 63.6 percent and 57.3 percent, and are superior to those (49.3 percent and 48.3 percent) of anabaena PD-1 (with a collection number of CCTCC No: M2011446). Thus, the anabaena PD-3 can be widely applied to the degradation of polychlorinated biphenyl in the environment, and has an important application prospect.

Description

technical field [0001] The invention relates to an Anabaena sp. PD-3 capable of efficiently degrading polychlorinated biphenyls and its application in microbial degradation of polychlorinated biphenyls. Background technique [0002] Polychlorinated biphenyls (PCBs), as an organic material with good chemical and thermal stability, have been widely used in industrial production fields. PCBs have obvious "three-induced" effects and strong fat solubility, and can accumulate and accumulate in organisms along the food chain, causing harm to organisms. Although its production and use were banned in 1979, due to its half-life of about 40 years, it has significant environmental persistence, and the semi-volatility of PCBs also makes it migrate around the world with atmospheric dry and wet deposition and eventually deposit in surface. Therefore, PCBs are commonly detected in polluted and non-polluted soils around the world. PCBs remaining in the environment have serious potential r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12A62D3/02C12R1/89A62D101/22
Inventor 张杭君蒋晓军刘嘉裕肖文丰鲁莉萍
Owner HANGZHOU NORMAL UNIVERSITY
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