Method for conducting fingerprint identification and analyzed object detection simultaneously through dark-field microscope

A dark field microscope and fingerprint recognition technology, applied in the fields of analyzing materials, measuring devices, human identification, etc., can solve problems such as unreported, complex and expensive large-scale instruments, and inability to achieve non-destructive analysis, and achieve the effect of easy standardization

Inactive Publication Date: 2014-04-23
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In summary, each of the existing technologies that have been adopted by the forensic field has its own shortcomings, and most importantly, the inability to detect chemical analytes in them outside of imaging; advanced technologies developed in recent years are able to detect analytes, but not

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  • Method for conducting fingerprint identification and analyzed object detection simultaneously through dark-field microscope
  • Method for conducting fingerprint identification and analyzed object detection simultaneously through dark-field microscope
  • Method for conducting fingerprint identification and analyzed object detection simultaneously through dark-field microscope

Examples

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Embodiment 1

[0028] Example 1: Aptamer-based Nanoplasmonic Probes Imaging Latent Fingerprints on Microscope Slides Under Dark Field Microscopy and Simultaneously Detecting Cocaine Carried in Fingerprints

[0029] (1) Preparation of nanoplasmonic probes: 150 mL trisodium citrate aqueous solution (2.2 mM) was heated to boiling for 15 min, and 1 mL chloroauric acid aqueous solution (25 mM) was added to react for 15 min. Cool down to 95°C, add 1mL chloroauric acid aqueous solution (25mM) to react for 30min, repeat twice. Then take out 55mL sample, and add 53mL water and 2mL trisodium citrate aqueous solution (60mM). Repeat the step of adding 1mL chloroauric acid aqueous solution (25mM) for reaction growth 6 times to obtain spherical gold nanoparticles Au NPs with a diameter of 50nm (this method can also prepare spherical gold nanoparticles with a diameter of 40-60nm, just change the last step to add 1 mL of chloroauric acid aqueous solution (25 mM) for the number of repetitions of the growth ...

Embodiment 2

[0036] Example 2: Aptamer-based nanoplasmonic probes image latent fingerprints on microscope slides under a dark-field microscope and simultaneously detect cocaine carried in the fingerprints

[0037] (1) Preparation of nanoplasmonic probes: Add chloroauric acid to 10 mL of cetyltrimethylammonium chloride aqueous solution (0.1 M) to a concentration of 0.25 mM. Add 0.45 mL of sodium borohydride aqueous solution (20 mM) and react at 30 °C for 1 hour as a seed solution. Prepare two bottles of growth solution: Add 320mg cetyltrimethylammonium chloride to 9.605mL deionized water, add 0.25mL chloroauric acid aqueous solution (10mM), 0.01mL sodium bromide aqueous solution (10mM), 0.09mL ascorbic acid Aqueous solution (40mM). React for 30min, repeat twice. Then take out 55mL sample, and add 53mL water and 2mL trisodium citrate aqueous solution (60mM). Add 0.45mL of the above seed solution to a bottle of growth solution and shake for 5 seconds, then take out 0.45mL and transfer it t...

Embodiment 3

[0041] Example 3: The nano-plasmonic probe based on the nucleic acid aptamer images the latent fingerprint on the microscope slide under a dark-field microscope and simultaneously detects the adenosine triphosphate carried in the fingerprint

[0042](1) Preparation of nanoplasmonic probes: 150 mL trisodium citrate aqueous solution (2.2 mM) was heated to boiling for 15 min, and 1 mL chloroauric acid aqueous solution (25 mM) was added to react for 15 min. Cool down to 95°C, add 1mL chloroauric acid aqueous solution (25mM) to react for 30min, repeat twice. Then take out 55mL sample, and add 53mL water and 2mL trisodium citrate aqueous solution (60mM). Repeat the step of adding 1mL chloroauric acid aqueous solution (25mM) for reaction growth 6 times to obtain spherical gold nanoparticles (Au NPs) with a diameter of 50nm (this method can also prepare spherical gold nanoparticles with a diameter of 40-60nm, just change the last Add 1 mL of chloroauric acid aqueous solution (25 mM) ...

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Abstract

The invention relates to a method for conducting fingerprint identification and analyzed object detection simultaneously through a dark-field microscope. The method comprises the following steps that a nanometer plasmon probe is made of a nanometer plasmon material and a nucleic acid aptamer; a substrate is processed and a latent fingerprint is obtained through the substrate; the nanometer plasmon probe is dropped to the substrate with the obtained latent fingerprint, so that the latent fingerprint is combined with the nanometer plasmon probe; the dark-field microscope is used for detection. According to the method for conducting fingerprint identification and analyzed object detection simultaneously through the dark-field microscope, the latent fingerprint can be imaged and chemical analyzed objects carried in the fingerprint can be detected simultaneously. A result shows that the method is simple and rapid in operation and can directly conduct nondestructive analysis on a fingerprint sample.

Description

technical field [0001] The invention relates to the technical field of fingerprint analysis and detection, in particular to a method for simultaneously performing fingerprint identification and analyte detection using a dark field microscope. Background technique [0002] Fingerprint analysis technology has been widely used in fields such as individual identification and forensic investigation. But so far, in practical applications, since latent fingerprints are not easy to be seen by the naked eye, they are extremely important and effective evidence left at the crime scene, so they are of great significance in forensic investigations. But so far, fingerprint analysis technology is only limited to the link of confirming personal identity with fingerprint characteristics. However, due to the complex and harsh conditions for processing latent fingerprints, the required instruments are bulky and expensive, and the direct detection of chemical analytes such as explosives and na...

Claims

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Application Information

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IPC IPC(8): A61B5/117G01N33/543
Inventor 樊春海李迪李昆
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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