Preparation method of efficient feed additive
A feed additive and high-efficiency technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of backward production technology, simple production equipment, single variety, etc., to improve the extraction yield, efficient extraction and utilization, improve extraction efficiency and quality effect
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Embodiment 1
[0061] Preparation of Bacillus subtilis culture:
[0062] Obtain Bacillus subtilis fermentation broth by step-by-step expansion culture of slant strains;
[0063] (1) Primary seed culture: Inoculate the slant strain of Bacillus subtilis into a 500 ml shake flask, with a medium capacity of 100 ml, a rotary shaker at 180 rpm, a culture temperature of 40°C, and a culture time of 12 hours;
[0064] (2) Secondary seed cultivation: Put the first-grade seeds into 500 ml second-grade seed shake flasks according to the inoculation amount of 10%, and the cultivation conditions are the same as the first-grade seeds;
[0065] (3) Tertiary seed cultivation: Inoculate the secondary seeds with 10% inoculum volume into a 5000 ml tertiary seed shaker flask, with a medium capacity of 1000 ml, a rotary shaker at 100 rpm, and a culture temperature of 40°C. Time 12 hours;
[0066] (4) First-level seed tank cultivation: put the third-level seeds into the first-level seed tank with a total volume ...
Embodiment 2
[0073] A high-efficiency feed additive comprises the following raw materials in parts by weight: 8 parts of Achyranthes bidentata polysaccharide, 5 parts of perilla extract, 4 parts of yeast cell wall extract, 32 parts of Bacillus subtilis culture, 4 parts of Astragalus extract; Codonopsis pilosula extract 6 copies; the preservation number of Bacillus subtilis Li-2013-02 is CGMCC No.7926.
Embodiment 3
[0075] The preparation method of the high-efficiency feed additive described in Example 2: the yeast cell wall extract is pulverized through a 60-mesh sieve, and the pulverized product is mixed with perilla extract, Bacillus subtilis culture, astragalus extract, Codonopsis pilosula extract, and Achyranthes bidentata polysaccharide The high-efficiency feed additive is obtained by mixing according to the proportion.
[0076] Preparation of astragalus extract:
[0077] Crush the raw material astragalus until the particle size is less than 2 mm, add 5 times the weight of water to the container and mix evenly, control the temperature at 85°C, keep it for 3 hours, adjust the pH value to 6.5 with lactic acid, cool down to 52°C, add mixed enzymes, enzymes After decomposing for 3 hours, add a mixture of ethanol and propanol which is 2 times the weight of the mixed material, control the temperature to 75° C., keep it for 4 hours, and filter; the filtrate is concentrated in vacuum and th...
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