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A method for male sterile line selection using the sterile cytoplasm of kenaf kn142

A KN142, male sterile line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problem of single cytoplasmic source of kenaf hybrids, and reduce the risk of a certain disease. low cost effect

Active Publication Date: 2015-09-16
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of a single cytoplasmic source of kenaf hybrids, and to provide a method for breeding a new type of kenaf cytoplasmic male sterile line, that is, a method for breeding male sterile lines using the sterile cytoplasm of kenaf KN142

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Breeding sterile lines with wild kenaf KN142

[0025] Taking the wild kenaf species KN142 as the female parent and Yuefeng No. 1 B (see: Example 3 of the National Invention Patent ZL200510019454.4) as the male parent, the method of saturated backcross was used to select the male sterile cytoplasm of KN142. The sterile line K4P3A and its maintainer line K4P3B.

[0026] details as follows:

[0027] From 2008 to 2010, crossed with KN142 as the female parent and Yuefeng No. 1 B as the male parent, and then backcrossed with Yuefeng No. 1 B as the male parent. At the same time of backcrossing, Fuhong 992 and R1 were used as the father. In this cross, the combining ability was determined. In each generation, the male sterile plants with high combining ability, similar traits to the male parent, and no anther dehiscence continued to be backcrossed, while the male parent was self-crossed. Backcrossing was carried out in Nanning, Guangxi and Lingshui, Hainan every yea...

Embodiment 2

[0028] Example 2 The sterile plant obtained by propagating offspring of KN142 seeds is used for transgenic sterile line

[0029] The seeds of natural pollination of wild kenaf species KN142 were harvested, and the seeds were planted to reproduce offspring, and sterile plants were obtained by screening. Taking the obtained sterile plant as the female parent and Yuefeng No. 1 B (see: Example 3 of the National Invention Patent ZL200510019454.4) as the male parent, the method of saturated backcrossing was used to select and breed the male sterile cytoplasm of KN142. The sterile line K4TP3A and its maintainer line K4TP3B.

[0030] details as follows:

[0031] From 2010 to 2012, the male sterile plant found in KN142 seed breeding offspring was used as the female parent, and Yuefeng No. 1B was used as the male parent to cross, and then backcrossed with Yuefeng No. 1B as the male parent. At the same time, Fuhong 992 and R1 were crossed as male parents to measure combining ability. I...

Embodiment 3

[0032] Example 3 Breeding sterile lines with wild kenaf KN142

[0033] Taking the wild kenaf species KN142 as the female parent and the conventional kenaf cultivar Jinguang Wuthorn (introduced from Fujian Agriculture and Forestry University) as the male parent, a sterile line with the cytoplasm of the KN142 male sterile line was selected by the method of saturation backcrossing. K4K3A and its maintainer K4K3B.

[0034] details as follows:

[0035] From 2010 to 2012, KN142 was used as the female parent, and Jinguang Wuthorn was used as the male parent to cross, and then backcross was performed with Jinguang Wuthorn as the male parent. At the same time of backcrossing, Fuhong 992 and R1 were crossed as the male parent, and the determination of Combining ability, select male sterile plants with high combining ability, similar traits to the male parent, and no anther dehiscence in each generation to continue backcrossing, while the male parent is self-crossed. Backcrossing was c...

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Abstract

The invention discloses a method for breeding a male sterile line by use of the sterile cytoplasm of hibiscus cannabinus KN142, which specifically comprises the following steps: performing saturation backcross by taking a breeding material containing the sterile cytoplasm of hibiscus cannabinus KN142 as a female parent and the cultivated variety of hibiscus cannabinus as a male parent, and selecting a uniform wild abortive type cytoplasmic male sterile line similar to the male parent in character and showing male sterility, wherein the male parent for backcross is a corresponding male sterility maintainer line. The method disclosed by the invention has the beneficial effects that the hybrid matched with the KN142 cytoplasmic male sterile line and the hybrid matched with the UG93 or H040 cytoplasmic male sterile line are popularized and utilized at the same time, and the occurrence risk of certain disease can be greatly reduced.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for breeding male sterile lines by utilizing the sterile cytoplasm of kenaf KN142. Background technique [0002] Kenaf, also known as "kenaf", is an annual bast fiber crop of the Malvaceae Hibiscus genus. Because kenaf is cultivated for the purpose of harvesting bast fibers or stalks, the heterosis rate of kenaf is as high as 35% to 40%. The utilization of kenaf heterosis has long been the main goal of kenaf breeding at home and abroad. After years of research, the Hemp Research Institute of the Chinese Academy of Agricultural Sciences invented the chemical tasseling hybrid seed production method of kenaf, and applied it in kenaf breeding. However, because kenaf is an infinite inflorescence, it needs to be sprayed several times, and the insect pollination is affected after the spraying, so manual pollination is required. In order to save costs, F has to be promoted in producti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 廖小芳赵艳红周瑞阳陈鹏周琼周步进
Owner GUANGXI UNIV
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