Functional beverage containing spirulina and skipjack bone extractive
A technology of extract and spirulina, applied in the field of functional beverages, can solve the problems of poor taste, low consumer acceptance, and unoptimistic market sales, and achieve the effects of stable quality, prevention or improvement of fatigue, and easy digestion.
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Embodiment 1-9
[0027] The functional beverage preparation technology of embodiment 1-9 spirulina bonito bone extract
[0028] 1. Materials and equipment
[0029] Spirulina: Spirulina was purchased from Hainan Diaisheng Microalgae Co., Ltd., 150g / box;
[0030] Skipjack bone extract: purchased from Lianhe Botai Biotechnology Co., Ltd., Dalian Free Trade Zone, 5kg / bag; the content of anserine is 9.83%.
[0031] Bromelain (enzyme activity 600GDU / g), β-cyclodextrin, xylitol, fumaric acid, natural mango flavor, PEG-400.
[0032] Water bath, pH meter, magnetic stirrer, spray dryer, filling machine, etc.
[0033] 2. Preparation process
[0034] (1) Weigh spirulina and bonito bone extract, add pure water and stir continuously to form a mixed solution;
[0035] (2) Add bromelain to the mixed solution in step (1), stir evenly, adjust the pH value and temperature of the solution, and obtain the hydrolyzed solution of spirulina and bonito bone extract that have been hydrolyzed;
[0036] (3) Add β-cy...
Embodiment 12
[0044] Example 12 Effects of Spirulina and Skipjack Bone Extract Combined on Uric Acid Concentration in Mouse Serum
[0045] 1. Experimental animals
[0046] 70 clean-grade Kunming mice (Slac: KM), weighing (20±2) g, were provided by Shanghai Kleiss Scientific Experimental Animal Center, license number: SCXK (Shanghai) 2008-0005. Adaptive feeding in our laboratory for 1 week before the experiment. Drinking water and standard pelleted diet were given ad libitum throughout the experiment.
[0047] 2. Experimental reagents and experimental equipment
[0048] Reagents: Beverages A (spirulina), B (hydrolyzate of spirulina and bonito bone extract), C (bonito bone extract), D (spirulina and bonito bone extract) prepared according to Examples 1, 3, 9, and 11 fish bone extract mixture); yeast paste, Beijing Aoboxing Biotechnology Co., Ltd., 500g / bottle; allopurinol tablets, Shanghai Xinyi Vientiane Pharmaceutical Co., Ltd., 0.1g / tablet.
[0049] Instruments: Beckman LX20 automatic ...
Embodiment 13
[0061] Acute toxicity and long-term toxicity test of embodiment 13 spirulina and skipjack bone extract composition
[0062] Take 60 clean-grade Kunming mice (Slac: KM), weighing (20±2) g, divide them into 6 groups of 10 mice, and give them the spirulina bonito bone extract drink prepared according to Table 4, respectively, according to each The volume of administration was calculated based on the weight of the animal before the first administration (gastric administration volume was 0.2ml / 10g); each administration was administered once at 7:00 am, 12:00 noon, and 5:00 pm for 14 consecutive days, and animal poisoning and death were recorded Case.
[0063] After the first oral administration, no obvious abnormalities appeared in the mice. After continuous gavage for 14 days, the mice did not show any poisoning reaction, and the eating, drinking, defecation and defecation were normal; no pathological changes were found in the internal organs after dissection. It is measured tha...
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