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Method for inhibiting Aspergillus fumigatus biofilm from formation

A technology of Aspergillus fumigatus and biofilm, applied in the biological field

Inactive Publication Date: 2014-04-09
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no drug that can effectively inhibit the formation of Aspergillus fumigatus biofilm has been found so far, so the development of new drugs that can effectively inhibit the formation of Aspergillus fumigatus biofilm is of great significance for the treatment of Aspergillus fumigatus biofilm-related infections

Method used

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  • Method for inhibiting Aspergillus fumigatus biofilm from formation
  • Method for inhibiting Aspergillus fumigatus biofilm from formation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Drug isochlorogenic acid A (National Institute for Food and Drug Control, batch number 110753-201314), dissolved in 100% dimethyl sulfoxide, made into a storage solution with a concentration of 90,000~100,000 μg / ml, and stored at -20°C after aliquoting store.

[0019] 2. Strains: 30-100 Aspergillus fumigatus strains were collected from September 2012 to May 2013 in the Bacteria Laboratory of the First Affiliated Hospital of Guangxi Medical University. The strain with the strongest ability to form biofilm was selected by the crystal violet method as the test strain. The quality control strain was Candida parapsilosis ATCC22019, which was donated to the Bacteria Laboratory of the First Affiliated Hospital of Guangxi Medical University.

[0020] 3. Carrier 1×1 cm 2 The polyvinyl chloride sheet (Haimen Shengbang Experimental Equipment Co., Ltd.) was soaked in 75% ethanol for 15 minutes, and then the carrier was soaked in sterile saline, and the ethanol on it was tho...

Embodiment 2

[0029] According to the results of the experiment done in Example 1, the drug susceptibility test results showed that the MIC of isochlorogenic acid A was >1000 μg / ml. Whether it was Aspergillus fumigatus at 24h or 48h, the mycelia of Aspergillus fumigatus in the blank group were all wrapped with dense extracellular matrix, and the extracellular matrix on the carrier at 48h was more, forming a typical Aspergillus fumigatus biofilm, while isochlorogenic acid In the group, only hyphae were seen, with little or no extracellular matrix, mainly showing the characteristics of planktonic bacteria. 24h inhibitory effect see figure 1 , 48h inhibitory effect see figure 2 .

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Abstract

The invention relates to a method for inhibiting a Aspergillus fumigatus biofilm from formation, belonging to the technical field of biology. The method comprises the following steps: preparing a drug, collecting a strain, preparing a carrier, preparing a spore suspension, carrying out drug sensitivity test, inhibiting an Aspergillus fumigatus biofilm, and scanning with an electron microscope, wherein the Aspergillus fumigatus biofilm inhibiting process comprises the following steps: adding prepared isochlorogenic acid A with different concentrations and a tested strain spore suspension into a 24-pore plate filled with the carrier, standing in a 35-37 DEG C biochemical incubator for culture, and taking out the carrier at the 24th hour and 48th hour and scanning with the electron microscope to observe the shape of the biofilm. Only hypha can be seen in the drug composed of the isochlorogenic acid A and spore suspension, and little and even no extracellular matrix can be seen, which indicates that the isochlorogenic acid A can inhibit the Aspergillus fumigatus biofilm from formation.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to aspergillus fumigatus biofilm, in particular to a method for inhibiting the formation of aspergillus fumigatus biofilm. Background technique [0002] Aspergillus fumigatus is an opportunistic pathogen that can cause severe infections in immunocompromised patients and can cause various diseases such as pulmonary aspergilloma, allergic bronchopulmonary aspergillosis, and invasive aspergillosis (IA). In recent years, the infection rate of Aspergillus fumigatus to immunocompromised patients is second only to Candida among the fungi, and with the increase of the number of immunocompromised patients, the incidence of invasive aspergillosis gradually increased. The investigation found that invasive aspergillosis is more common in severe Intensive care unit (ICM) morbidity increased from 0.3% to 5.8%, and the mortality rate was as high as 30-100%. The drugs that can be used clinically to treat in...

Claims

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Application Information

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IPC IPC(8): C12Q1/02A61K31/216A61P31/10C12R1/68C12R1/72
Inventor 陈一强黄宏张瑾珏孔晋亮
Owner GUANGXI MEDICAL UNIVERSITY
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