Lepista sordida first-class strain solid culture medium and strain rapid cultivating method
A solid culture medium, the technology of fragrant mushroom, applied in the directions of botanical equipment and method, fertilizer mixture, horticulture, etc., can solve the problems of low conversion rate of fragrant mushroom, long production cycle of fragrant mushroom, etc., and achieve dense mycelium. Thick, high success rate, saving manpower and material resources
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Embodiment 1
[0022] The solid culture medium of the first-grade strains of the mushroom, calculated by weight, includes 185 parts of flour, 26 parts of pine needles, 0.20 parts of potassium dihydrogen phosphate, 0.05 parts of magnesium sulfate, 0.35 parts of peptone, 18 parts of white sugar, and 15 parts of agar 1000ml of water, the preparation method is: mix the above-mentioned components evenly, heat to boiling, after the agar is completely dissolved, filter with gauze, pack the above-mentioned solution in conical flasks, and seal it with a silica gel stopper. Under the conditions of 120-125℃ and 0.15MPa, sterilize the Erlenmeyer flask for 25-40min, and pour the sterilized culture medium into the flat plate in the ultra-clean workbench to obtain the solid culture medium of the first-grade strain;
[0023] The rapid cultivation method of strains is characterized in that: inoculate the mother species of Amanita chinensis on the solid medium, keep the temperature at 20-28°C, and cultivate in...
Embodiment 2
[0025] The solid culture medium of the first-grade strains of fenugreek mushrooms, calculated in parts by weight, includes 220 parts of flour, 34 parts of pine needles, 0.45 parts of potassium dihydrogen phosphate, 0.2 parts of magnesium sulfate, 0.7 parts of peptone, 30 parts of white sugar, and 30 parts of agar 1000ml of water, the preparation method is: mix the above-mentioned components evenly, heat to boiling, after the agar is completely dissolved, filter with gauze, pack the above-mentioned solution in conical flasks, and seal it with a silica gel stopper. Under the conditions of 120-125°C and 0.15MPa, sterilize the Erlenmeyer flask for 25-40 minutes, and pour the sterilized culture medium into a flat plate in an ultra-clean workbench to obtain a solid culture medium for the first-grade strain.
[0026] The rapid cultivation method of strains is characterized in that: inoculate the mother species of Amanita chinensis on the solid medium, keep the temperature at 20-28°C, ...
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