Lepista sordida first-class strain solid culture medium and strain rapid cultivating method

A solid culture medium, the technology of fragrant mushroom, applied in the directions of botanical equipment and method, fertilizer mixture, horticulture, etc., can solve the problems of low conversion rate of fragrant mushroom, long production cycle of fragrant mushroom, etc., and achieve dense mycelium. Thick, high success rate, saving manpower and material resources

Inactive Publication Date: 2014-04-09
贵州尚恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no industrialized companies and individuals to transform the mushrooms. The main reasons for this are the following two problems: (1) The transformation rate of the mushrooms is low, only 14-20%, compared to other edible mushrooms. As far as the conversion rate of fungi is 80-150%, further technical research is needed; (2) The production cycle of the flower face mushroom is long. Under artificial climate conditions, the indoor temperature is controlled at 20-28°C. It takes 90 days for the bag to be full of mycelium, plus the time for seed production and fruiting, it takes 150 days of working time

Method used

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  • Lepista sordida first-class strain solid culture medium and strain rapid cultivating method
  • Lepista sordida first-class strain solid culture medium and strain rapid cultivating method
  • Lepista sordida first-class strain solid culture medium and strain rapid cultivating method

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Embodiment 1

[0022] The solid culture medium of the first-grade strains of the mushroom, calculated by weight, includes 185 parts of flour, 26 parts of pine needles, 0.20 parts of potassium dihydrogen phosphate, 0.05 parts of magnesium sulfate, 0.35 parts of peptone, 18 parts of white sugar, and 15 parts of agar 1000ml of water, the preparation method is: mix the above-mentioned components evenly, heat to boiling, after the agar is completely dissolved, filter with gauze, pack the above-mentioned solution in conical flasks, and seal it with a silica gel stopper. Under the conditions of 120-125℃ and 0.15MPa, sterilize the Erlenmeyer flask for 25-40min, and pour the sterilized culture medium into the flat plate in the ultra-clean workbench to obtain the solid culture medium of the first-grade strain;

[0023] The rapid cultivation method of strains is characterized in that: inoculate the mother species of Amanita chinensis on the solid medium, keep the temperature at 20-28°C, and cultivate in...

Embodiment 2

[0025] The solid culture medium of the first-grade strains of fenugreek mushrooms, calculated in parts by weight, includes 220 parts of flour, 34 parts of pine needles, 0.45 parts of potassium dihydrogen phosphate, 0.2 parts of magnesium sulfate, 0.7 parts of peptone, 30 parts of white sugar, and 30 parts of agar 1000ml of water, the preparation method is: mix the above-mentioned components evenly, heat to boiling, after the agar is completely dissolved, filter with gauze, pack the above-mentioned solution in conical flasks, and seal it with a silica gel stopper. Under the conditions of 120-125°C and 0.15MPa, sterilize the Erlenmeyer flask for 25-40 minutes, and pour the sterilized culture medium into a flat plate in an ultra-clean workbench to obtain a solid culture medium for the first-grade strain.

[0026] The rapid cultivation method of strains is characterized in that: inoculate the mother species of Amanita chinensis on the solid medium, keep the temperature at 20-28°C, ...

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Abstract

The invention discloses a lepista sordida first-class strain solid culture medium and a strain rapid cultivating method. A preparation method of the culture medium comprises the following steps: uniformly mixing flour, pine needle, monopotassium phosphate, magnesium sulfate, peptone, white granulated sugar, agar and water, heating to boil, sterilizing a conical flask filled with the culture medium for 25-40 minutes at the temperature of 120-125 DEG C and under the pressure of 0.15Mpa, and pouring the culture medium obtained after the sterilization into a flat plate in a super clean bench to obtain the lepista sordida first-class strain solid culture medium. By adopting the technical scheme, the lepista sordida first-class strain is vigorous in growth, dense, thick and strong in mycelium, many in aerial mycelium and short in growth cycle; the activity of the strain is effectively guaranteed; lots of manpower and material resources are saved for industrial artificial cultivation and breeding of lepista sordida.

Description

technical field [0001] The invention relates to the field of agricultural cultivation science and technology, in particular to a solid culture medium for first-class strains of A. chinensis and a rapid cultivation method for the strains. Background technique [0002] Lepista sordida (Schum.: Fr.) Sing is a large fungus belonging to the order Agaricaceae, the family Agaricaceae, and the genus Lepista sordida. Because of the water-soaked pattern on the edge of the cap, it is called flower face mushroom, purple flower face, purple golden mushroom, etc. It is a delicious and nutritious edible and medicinal fungus. Flower face mushroom is rich in nutrition. It is determined that every 100 grams of fresh mushrooms contains 2.9 grams of protein, 0.2 grams of fat, 3 grams of carbohydrates, 0.6 grams of crude fiber, 8 mg of calcium, 6.6 mg of phosphorus, 1.3 mg of iron, and 4 mg of vitamin C. Niacin 3.3 mg, in addition to sodium, potassium, manganese, copper, zinc, fluorine, iodine,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
Inventor 向准王文祥于飞王寒秋罗倩孙超王莹贺红早任春光
Owner 贵州尚恩生物科技有限公司
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