Preparation method of pig spleen transfer factor

Abstract

The invention provides a preparation method of a pig spleen transfer factor. The preparation method comprises the steps of tissue mincing, breaking by homogenizing, microfiltration, ultrafiltration and the like. A high-pressure homogenizer is adopted to rapidly break cells and can effectively keep the activity of the transfer factor; besides, compared with the prior art, the technical scheme provided by the invention does not need a freezing-thawing step, therefore, the production period is shortened; the microfiltration-ultrafiltration technology is adopted to intercept effective constituents with appropriate molecular weight, so as to obtain a transfer factor stock solution, and compared with the traditional dialysis technology, the microfiltration-ultrafiltration technology is relatively high in efficiency. The method provided by the invention is suitable for scale production requirement, and the production period can be greatly shortened; the transfer factor solution prepared from the healthy pig spleen as a raw material through defatting, cell breakage, microfiltration and ultrafiltration is non-specific immunoregulator and is used for enhancing the immunological function of animal bodies.

Description

Technical field [0001] The invention relates to the field of veterinary biological products, in particular to a method for preparing pig spleen transfer factor. Background technique [0002] Transfer factor (TF) is a substance released by lymphocytes that can transfer sensitization information. It can specifically transfer the donor’s cellular immune information to the recipient, thereby enhancing the recipient’s immune function. It has become a widely used immune enhancement agent abroad. [0003] The transfer factor exists in the lymphocytes of the spleen. The transfer factor preparation method in the prior art generally involves crushing the animal spleen, destroying the cell structure to release the intracellular transfer factor into the serum, and then separating and purifying the transfer factor in the serum. Finally, the transfer factor product is obtained. [0004] The composition of animal spleen slurry is complex, so animal TF is not easy to separate and purify; the molec...

AI Technical Summary

Problems solved by technology

[0008] It can be found from the above technical solutions of the prior art that the dialysis method refers to performing several times of dialysis on the supernatant of the homogenate of the animal spleen to realize the separation and purification of the transfer factor. This method is simple to operate, but the production cycle is long and the operation cost is high. , it is difficult to carry out large-scale industrial production; the filtration method is to crush and homogenize the spleen tissue first, and then centrifuge to obtain the supernatant for filtration to realize the separation and purification of the transfer factor. The crushing steps of this method are generally divided into primary tissue crushing and freezing. Thawing and crushing, in which the freeze-thaw crushing step takes a long time, and the initial crushing often has the phenomenon of incomplete cell crushing due to the limitation of crushing equipment