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Oncoprotein P185 detection kit

A detection kit and tumor protein technology, applied in the field of tumor protein P185 detection kits

Active Publication Date: 2014-04-02
ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
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Problems solved by technology

Compared with the detection of the expression of tumor protein P185 in tumor tissue, the detection of the expression of tumor protein P185 in the patient's serum is simple and easy, and is suitable for clinical needs. However, there is no kit for detecting P185 in the serum of patients in China, so a highly sensitive kit has been developed. The highly specific, highly specific and stable P185 detection kit is used for the detection of P185 in the serum of more than ten kinds of cancers such as breast, ovary, lung, stomach, liver, etc., which is of great significance for the screening, diagnosis and treatment of the above tumors

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Embodiment Construction

[0019] The following is a further description of the preparation, use steps and use effects of the tumor protein P185 detection kit:

[0020] 1. Preparation of tumor protein P185 detection kit

[0021] 1) Preparation of routine equipment and reagents:

[0022] 96-well ELISA plate, P185 positive serum, P185 negative serum, washing solution, stop solution, horseradish peroxidase-streptavidin, chromogenic solution A, chromogenic solution B, of which P185 positive serum and P185 negative serum The compliance rate is 100%.

[0023] 2) Preparation of monoclonal antibody against tumor protein P185:

[0024] a) Collect NIH / 3T3 cells to i.p immunize the first batch of Balb / C female mice for 6-8 weeks, the dose of NIH / 3T3 cells is 10 7 / mouse, once every two weeks, blood was taken from the tail of the female mouse three times later, and the serum titer was measured by ELISA method, and the female mouse with the highest titer was immunized with the same amount of NIH / 3T3 cells for i.p...

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Abstract

The invention discloses an oncoprotein P185 detection kit. The oncoprotein P185 detection kit comprises oncoprotein P185 monoclonal antibodies, an elisa plate, a positive reference substance, a negative reference substance, cleaning liquor, a stop buffer, horse radish peroxidase-chain avidin and a developing substrate thereof, wherein the oncoprotein P185 monoclonal antibodies comprise an oncoprotein P185 monoclonal antibody enveloped on the elisa plate and the oncoprotein P185 monoclonal antibody marked by biotin. The oncoprotein P185 detection kit disclosed by the invention can be adopted for effectively and stably measuring oncoprotein P185 level in human serum, is high in sensitivity and precision, and good in test repeatability.

Description

technical field [0001] The invention relates to the fields of biology and medical examination, in particular to a tumor protein P185 detection kit. Background technique [0002] P185 is an important tumor surface marker protein encoded by the oncogene HER-2 / neu. It is overexpressed in more than ten kinds of cancer patients such as breast, ovary, lung, stomach, and liver. The tumors of patients with overexpressed P185 The degree of malignancy is high, postoperative metastasis and recurrence are prone to occur, and it is resistant to chemotherapy drugs. Therefore, the detection of the expression level of tumor antigen P185 is of great significance for the differential diagnosis of various tumors, classification, prediction of curative effect, and selection of treatment options. , especially in breast cancer, P185 has been internationally recognized as an important clinical indicator. Compared with the detection of the expression of tumor protein P185 in tumor tissue, the dete...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/57415G01N33/57446G01N33/6893G01N2800/7028
Inventor 宋礼华徐振山周业亭吴强
Owner ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
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