A method of hydrothermal pretreatment to increase the production of ethanol from kitchen waste
A technology for food waste and hydrothermal treatment, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex composition of biological bacteria liquid and mixed enzyme preparation, difficult acquisition of bacteria, cumbersome configuration methods, etc. , to achieve good application value, low production cost and high raw material utilization
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Embodiment 1
[0034] 1. Preliminarily sort the collected food waste, remove the sundries that are not easy to ferment, put it into the hydrothermal reactor, add 1 times the volume of water, and conduct hydrothermal treatment at 140°C for 50 minutes, during which time, turn on the motor to stir for 20 minutes.
[0035] 2. After the hydrothermal treatment, spray the kitchen waste to the three-phase separator, and separate the grease after standing still.
[0036]3, water and solid are sent to fermentation tank, add the composite enzyme of 0.5% of solid weight, the mass ratio of various enzymes in composite enzyme is amylase: cellulase: lipase: protease: glucoamylase: xylanase= 2:0.8:0.6:1.3:1:2.5. The composition of the enzyme preparation is: amylase (200000u / g), cellulase (200000u / g), lipase (200000u / g), protease (200000u / g), glucoamylase (100000u / g), xylanase (100000u / g). Then add 10% composite flora of solid weight, and ferment for 96 hours at 32°C. The ratio of each species in the comp...
Embodiment 2
[0038] The main steps of this embodiment are the same as in Example 1, the difference is that in step 1, when the food waste contains more water, after preliminary sorting to remove impurities that are not easy to ferment, it is directly hydrothermally treated at 200°C for 10 minutes, and at the same time Do not turn on the stirring motor. After the hydrothermal treatment, the pressure is released. When the pressure of the hydrothermal reactor is released to 1.3atm, the kitchen waste is sprayed into the three-phase separator, and after standing still, the oil and the water exceeding the solid-liquid ratio range required for ethanol fermentation are separated. The remaining solids and water were sent to the fermenter, and 0.05% of the solid weight of the compound enzyme and 35% of the solid weight of the mixed flora were added, and fermented at 35°C for 130 hours.
Embodiment 3
[0040] The main steps of this embodiment are the same as in Example 1, the difference is that in step 3, the kitchen waste water stored in three-phase separation is added so that the solid-liquid volume ratio is 1:6, and then 2% of the solid weight of the compound enzyme and the solid weight are added 0.3% of the complex flora. Wherein, the mass ratio of each enzyme in the compound enzyme is amylase: cellulase: lipase: protease: glucoamylase: xylanase=0.5:5:1:2:2:1. The ratio of each bacterial species in the composite flora is Kluyveromyces: Saccharomyces cerevisiae: Pseudomonas saccharophila: Bacillus subtilis: Aspergillus niger: Rhizopus oryzae: Trichoderma konii: Mucorella ruckeri=7: 1:5:1:1:6:1:1. Fermentation was carried out at 43°C for 96 hours.
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