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Structure grease containing eicosatetraenoic acid and preparation method thereof

A technology of eicosatetraenoic acid and structured oils, which is applied in the field of edible oils and fats and can solve the problems of low conversion efficiency

Inactive Publication Date: 2014-04-02
RUNKE BIOENG FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In normal human body, arachidonic acid (ARA) can be obtained through partial conversion of linoleic acid, but the conversion efficiency of this pathway is extremely low, and must be catalyzed by a series of enzymes

Method used

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  • Structure grease containing eicosatetraenoic acid and preparation method thereof
  • Structure grease containing eicosatetraenoic acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In the present embodiment, the preparation method of structured grease comprises the following steps:

[0028] (1) Enzymatic transesterification of raw oil and eicosatetraenoic acid donor in the presence of specific Sn-1,3 lipase;

[0029] The aforementioned raw oil is lard, the donor of eicosatetraenoic acid is ethyl eicosatetraenoate, and the weight ratio of the donor of eicosatetraenoic acid to the raw oil is 2:1.

[0030] During the reaction, the raw oil and the eicosatetraenoic acid donor are mixed, and stirring can be carried out during the reaction to make the mixing more complete.

[0031] In this step (1), the dosage of specific Sn-1,3 lipase is 5% of the total weight of raw oil and eicosadonic acid donor, the reaction temperature is 70° C., and the reaction time is 3 hours.

[0032] (2) After the enzymatic transesterification reaction, removing the specific Sn-1,3 lipase to obtain an intermediate product;

[0033] In this step (2), the specific Sn-1,3 lipase...

Embodiment 2

[0039] In the present embodiment, the preparation method of structured grease comprises the following steps:

[0040] (1) Enzymatic transesterification of raw oil and eicosatetraenoic acid donor in the presence of specific Sn-1,3 lipase;

[0041] The above-mentioned raw material oil is palm oil, the donor of eicosatetraenoic acid is ethyl eicosatetraenoate, and the weight ratio of the donor of eicosatetraenoic acid to the raw oil is 2:1.

[0042] During the reaction, the raw oil and the eicosatetraenoic acid donor are mixed, and stirring can be carried out during the reaction to make the mixing more complete.

[0043] In this step (1), the dosage of specific Sn-1,3 lipase is 8% of the total weight of raw oil and eicosadonic acid donor, the reaction temperature is 60° C., and the reaction time is 3 hours.

[0044] (2) After the enzymatic transesterification reaction, removing the specific Sn-1,3 lipase to obtain an intermediate product;

[0045] In this step (2), the specific...

Embodiment 3

[0051] In the present embodiment, the preparation method of structured grease comprises the following steps:

[0052] (1) Enzymatic transesterification of raw oil and eicosatetraenoic acid donor in the presence of specific Sn-1,3 lipase;

[0053] The above-mentioned raw material oil is lard, and the donor of eicosatetraenoic acid is a mixture of methyl eicosatetraenoate and ethyl eicosatetraenoate (wherein methyl eicosatetraenoate and eicosatetraenoate The weight of ethyl enoate accounts for half each), and the weight ratio of eicosatetraenoic acid donor and raw oil is 3:1.

[0054] During the reaction, the raw oil and the eicosatetraenoic acid donor are mixed, and stirring can be carried out during the reaction to make the mixing more complete.

[0055] In this step (1), the dosage of the specific Sn-1,3 lipase is 1% of the total weight of the raw oil and the eicosadonic acid donor, the reaction temperature is 75° C., and the reaction time is 6 hours.

[0056] (2) After the...

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Abstract

The invention provides structure grease containing eicosatetraenoic acid. In the structure grease, eicosatetraenoic acid at the Sn-1,3 site accounts for over 40% (in terms of weight) of total fatty acid, and palmitic acid at the Sn-2 site accounts for over 50% (in terms of weight) of total palmitic acid. The invention also provides a preparation method of the structure grease. The structure grease disclosed by the invention contains eicosatetraenoic acid, eicosatetraenoic acid at the Sn-1,3 site and palmitic acid at the Sn-2 site are easily digested and absorbed by a human body, eicosatetraenoic acid is supplemented when palmitic acid at the Sn-2 site is provided, and the humanization direction of fat is met. Therefore, the structure grease can be applied to infant formula food, and other foods or health products requiring eicosatetraenoic acid.

Description

technical field [0001] The invention relates to edible oils and fats, in particular to a structural oil rich in eicosadonic acid and a preparation method thereof. Background technique [0002] Fat is fat or triglyceride (triglyceride for short), which is formed by the esterification of glycerol and three fatty acids. The fatty acid in the middle is called Sn-2 fatty acid, and the fatty acid in both ends is called Sn-1. 3 fatty acids. Structured fats are fats with specific fatty acids in specific positions. [0003] Fatty acids in human milk fat have a highly specific location distribution on the glycerol backbone, and this specific structure is of great significance for the effective absorption of nutrients. Palmitic acid (C16:0) is the main saturated fatty acid in human milk, accounting for 20-25% of the total fatty acids in human milk, of which 70-75% of palmitic acid is esterified at the Sn-2 position of triglycerides. [0004] The human pancreatic lipase system proces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 李建平任国谱
Owner RUNKE BIOENG FUJIAN
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