Method for preparing armillaria mellea strains by using cut logs

Abstract

The invention belongs to the technical field of strain cultivation and relates to a method for preparing armillaria mellea strains by using cut logs. The method comprises the steps: (A) blending materials: weighing culture materials according to a formula of a culture medium, mixing uniformly, and adding water until the water content is 55-70%, wherein the culture medium is prepared from the following ingredients in parts by weight: 8-12 parts of dry branch segments of weed-tree branches, 12-18 parts of wheat bran, 8-12 parts of maize flour, 2-4 parts of soybean meal, 1 part of white sugar, 1 part of gypsum powder and 1 part of potassium dihydrogen phosphate; (B) bagging the uniformly mixed culture materials, sterilizing and inoculating; (C) culturing: culturing the inoculated material bags at the temperature of 20-25 DEG C until the material bags are full of armillaria mellea hyphae. According to the method, the culture medium is appropriate in ingredient and ratio and balanced in nutrition; during the preparation of the armillaria mellea hyphae, the culture medium is strong in water retention and sufficient in nutrition, and the obtained armillaria mellea hyphae are thick and strong and are high in survival ratio and yield.

Description

technical field [0001] The invention belongs to the technical field of strain cultivation, and relates to a method for making armillaria strains by utilizing segment wood. Background technique [0002] Armillaria mellea (Armillaria mellea), also known as Armillaria mellea, honey-colored fungus, honey mushroom, belongs to the fungal phylum, basidiomycotina, layer fungi, non-compartment basidiomycete subclass, agaricales, Tricholomaceae, honey ring Fungus, is a kind of medicinal and edible fungus. The fruiting body is generally medium-sized; the diameter of the cap is 4-14cm, light khaki, honey-colored to light yellow-brown; when old, it is brown, with flat or erect small scales in the middle, sometimes nearly smooth, with stripes on the edge; the flesh is white; The gills are white or slightly fleshy pink, and dark brown spots often appear when old; the stipe is slender, cylindrical, slightly curved, the same color as the cap, fibrous, soft and hollow inside, and slightly en...

AI Technical Summary

Problems solved by technology

However, as far as the production technology of Armillaria miscella is concerned, the disclosed medium components and preparation methods cannot achieve ideal results in actual production. The outstanding shortcoming is that the nutrition of the medium is unbalanced, and the bacteria Low seed survival rate, low yield, and poor water retention of wood segments