Method for determining soil contamination condition through crop markers

Abstract

The invention relates to a method for determining soil contamination condition through crop markers. The method is characterized by comprising the following steps: taking maize seedlings as a study crop; after the maize seedlings grow for 14d, taking down maize leaves to prepare a crude enzyme liquid; testing the activity of ascorbate peroxidase in the maize leaves, and measuring the content of reduced glutathione in the maize leaves; when the activity of the ascorbate peroxidase and the content of the reduced glutathione change remarkably, determining that the soil is contaminated. When the soil is contaminated, the cadmium concentration is greater than or equal to 0.3 mg / kg. The method is low in cost and simple in operation; responses to low- concentration contaminants are sensitive; the method can be comprehensively applied to soil pollution condition diagnosis and quality evaluation.

Description

technical field [0001] The invention relates to a method for detecting soil pollution using crop markers, in particular to a technique for evaluating soil pollution based on the dose-effect response of soil pollutants and corn seedling biomarkers. Background technique [0002] Studies have reported that my country's heavy metal-contaminated soil exceeds 300 million mu, accounting for about 1 / 6 of the total cultivated land (Ye Changming et al., 2004). Moreover, with the development of industry and agriculture, the area and degree of soil heavy metal pollution are bound to show an increasing trend. The accumulation of heavy metals in the soil to a certain extent will cause toxicity to the soil-plant system, which not only leads to the degradation of land quality, affects the safety of agricultural products, and poses a serious threat to human and animal health through the food chain, but also pollutes the surface through runoff and leaching water and groundwater, deterio...

AI Technical Summary

Problems solved by technology

However, the traditional method of judging soil pollution based on the growth and development of terrestrial higher plants is susceptible to interference from plant growth cycles and other external factors, and is not suitable for rapid diagnosis of soil pollution.

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