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New application of phytosterol functioning as elastinase activity inhibitor, and composition containing the same

A kind of technology of elastase and activity inhibitor, applied in the field of phytosterol and its composition as an active ingredient

Inactive Publication Date: 2014-03-26
COTDE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present inventors searched for anti-aging substances that can improve the skin elasticity weakening caused by the reduction and destruction of elastin and solve the problems of skin safety and stability when blending cosmetics with plant-based natural products, and found that phytosterols show Good elastase inhibitory effect, also can obviously improve skin elasticity and significantly promote anti-aging effect and skin wound healing effect when being coated on people's skin, thereby completed the present invention

Method used

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  • New application of phytosterol functioning as elastinase activity inhibitor, and composition containing the same
  • New application of phytosterol functioning as elastinase activity inhibitor, and composition containing the same
  • New application of phytosterol functioning as elastinase activity inhibitor, and composition containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] [Example 1] Production of phytosterols from soybean oil deodorized distillates

[0026] Pour 200g of soybean oil deodorized distillate (phytosterol content: 27%) and 200g of toluene into a 3L three-necked flask, stir at 300rpm and heat up to 90°C, then add 200g of 25% (w / w) NaOH solution. Thereafter, the reaction product was stirred at 300 rpm at 80° C., 150 g of a 50% (w / w) sulfuric acid solution was slowly added, stirred for 15 minutes, and left to stand to remove the water layer to obtain an oil layer. The oil layer was washed repeatedly until the sulfuric acid was completely removed, and then filtered under reduced pressure at 110°C to remove toluene. Mix 300g of methanol on the above filtrate, pour it into a 3L three-neck flask, react at 65°C for 30 minutes, cool to room temperature, and stir for 24 hours, filter the fixed product generated during the stirring process, and wash it with 200g of methanol , the solid product is distilled under reduced pressure to com...

Embodiment 2

[0027] [Example 2] Isolation and Confirmation of Phytosterols

[0028] 1.5 g of the above-mentioned phytosterols were separated by C18 column chromatography. At this time, the used column eluate starts with a solvent in which methanol and water are each mixed in a volume ratio of 80:20 (v / v), and gradually increases the ratio of methanol within 40 minutes so that methanol becomes 100%. The speed and detection wavelength are 60ml / min and UV 210nm respectively. After the above C18 column chromatography separation, 0.75 g of β-sitosterol and 0.38 g of stigmasterol were obtained from subfractions 48-52 and 55-60.

[0029] The instrumental analysis results of the separated phytosterols are as follows.

[0030] Compound 1 (β-sitosterol)

[0031] 1 H-NMR (CDCl 3 , 600MHz) δ3.53(tdd, 1H, J=4.5, 4.2, 3.8Hz, H-3), δ5.36(t, 1H, J=6.4Hz, H-5), δ0.93(d, 3H , J=6.5Hz, H-19), δ0.84(t, 3H, J=7.2Hz, H-24), δ0.83(d, 3H, J=6.4Hz, H-26), δ0.81 (d, 3H, J=6.4Hz, H-27), δ0.68(s, 3H, H-28), δ1...

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Abstract

The invention is designed based on the elastinase deactivation research for phytosterols and relates to the new application of a phytosterol functioning as an elastinase activity inhibitor, and an external-use agent composition containing the same and capable of improving the skin elasticity and healing skin wounds. The phytosterol-containing combination can be used safely without any side effect on the skin. Meanwhile, by means of the composition, the skin elasticity is improved through deactivating the elastase, so that the skin anti-aging effect and the skin wound healing effect are greatly enhanced.

Description

technical field [0001] The present invention relates to a phytosterol functioning as an elastase activity inhibitor and a composition containing the phytosterol as an active ingredient. The composition can improve skin elasticity by inactivating elastase and greatly enhance skin anti-aging effect and skin wound healing effect . Background technique [0002] In the skin, elastin fibers and collagen fibers together form cross-links inside the dermis (epiderm) to provide skin elasticity. As the age increases, the skin shows the phenomenon of lack and aggregation of elastin fibers, decrease of collagen fibers, and increased infiltration of inflammatory cells histologically, and biochemically, the activity of elastase, which is an elastin-decomposing enzyme, significantly increases The phenomenon. Therefore, the action of elastase causes the skin elasticity to decrease sharply, resulting in sagging. Elastase is the only enzyme discovered so far that breaks down elastin, and in...

Claims

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Application Information

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IPC IPC(8): A61K8/63A61K31/575A61Q19/08A61P17/02
CPCA61Q19/00A61K8/63A61K9/0014A61K31/575
Inventor 具昌燮金珉珍郑伦朱张东逸
Owner COTDE
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