Probiotics-containing infant formula, and preparation method and storage method thereof
A technology of infant formula and active probiotics, which is applied in the field of infant formula milk powder and preparation, and can solve problems such as unstable product quality, decreased number of live bacteria in milk powder, and easy loss of activity of probiotics
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preparation example Construction
[0041] Preparation of normal saline and medium: Prepare normal saline: dissolve 8.5g of sodium chloride in 1000ml of distilled water, autoclave at 120°C / 15min; MRS agar medium (purchased from Merck, Germany), autoclave at 120°C / 15min.
[0042] equipment:
[0043] Clean Bench The Baker Company SG403
[0044] Anaerobic Incubator Don Whitley Scientific A35
[0045] Electric constant temperature incubator INE500
[0046] Autoclave HVE-50
[0047] Electronic balance FA2104
[0048] Water Activity Meter AQUALAB Series4
[0049] Moisture Meter MB45
Embodiment 1
[0050] The impact of embodiment 1 temperature factor on infant formula milk powder probiotic live bacteria number
[0051] Infant formula milk powder was prepared according to conventional methods in the art, and the formula of infant formula milk powder is shown in Table 1.
[0052] Table 1 Formula of infant formula milk powder (calculated per 100 grams of milk powder)
[0053]
[0054]
[0055] The resulting infant formula has a water activity of a w 0.18, the moisture content is 1.8%, please refer to the instrument instruction manual for the detection method of water activity and moisture content.
[0056] Table 2 Nutrient composition of infant formula milk powder (calculated per 100 grams of milk powder)
[0057] nutrient content
unit
content
g
8.2
g
1.8
g
18
Demineralized Whey Powder
g
9
whey protein concentrate
g
9
g ...
Embodiment 2
[0075] Example 2 The influence of water activity factors on the number of live bacteria of probiotics in infant formula milk powder
[0076] (1) Preparation of follow-on formula and toddler formula
[0077] According to the preparation method described in Example 1, older infant formula milk powder is prepared, and the formula of older infant formula milk powder is as shown in Table 5:
[0078] Table 5 Formula of formula milk powder for older infants (calculated per 100 grams of milk powder)
[0079]
[0080]
[0081] The resulting follow-on formula has a water activity of a w 0.21, moisture content is 2.0%, and the nutrient composition of described older infant formula milk powder is shown in Table 6.
[0082] Table 6 Nutrient composition of formula milk powder for older infants (calculated per 100 grams of milk powder)
[0083] nutrient content
unit
content
g
17.8
G
2.2
g
8 ...
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