Medium for producing ivermectin by fermenting streptomyces avermitilis and fermenting method

Abstract

The invention relates to a medium for producing ivermectin by fermenting streptomyces avermitilis and a fermenting method. By adding substances such as formula molasses, formula earthworm powder and the like into the medium for optimizing the medium formula, and corresponding optimizing the fermenting technology, the medium and the cultivation method for enabling ivermectin fermentation unit to be stable are provided, the average fermentation unit of ivermectin is 6000 mu g / mL or more and is improved by 20% or more compared with domestic conventional fermentation level. The fermentation period of ivermectin is averagely shortened by 10 h or more, so that the problem is effectively solved that conventional fermentation technology is high in cost; and the environmental influence on sources of raw materials and auxiliary materials is furthest reduced, and the raw materials and the auxiliary materials are guaranteed to be sufficiently supplied, so that the stable efficient production of ivermectin is realized.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular to a culture medium and a fermentation method for fermenting and producing ivermectin by Streptomyces avermanii. Background technique [0002] my country is one of the largest pesticide production countries in the world, and it is also one of the largest pesticide export countries. my country's pesticide industry has developed very rapidly, the output of pesticides has almost doubled, and the varieties of pesticides have almost doubled. With the continuous deepening of pesticide creation and the continuous improvement of the research and development level, some new pesticide varieties and bio-pesticides have begun to be exported to foreign countries. The discovery of avermectin biopesticides opened a new era of anti-helminth drug research, as broad-spectrum insecticides and molluscicides have a wide range of uses in the fields of medicine, agriculture, and animal hu...

AI Technical Summary

Problems solved by technology

[0003] At present, the production process of ivermectin reported at home and abroad mainly contains two aspects: one is the homogeneous catalytic selective hydrogenation of abamectin and prepared, the catalyst system used is a hydrocarbon-soluble Wilkinson catalyst, and the organic solvent involved is mainly toluene or benzene, etc., and this method has problems such as low synthesis yield, high production cost, heavy metals and environmental pollution; another The first is a genetically engineered bacterium that uses ivermectin, and uses secondary fermentation or tertiary fermentation to produce ivermectin. There are major problems in this method:

[0004] 1) Using the conventional medium formula, the fermentation unit is unstable, and the fermentation level fluctuates in the range of 3000-5000ug / ml, resulting in large differences in the fermentation level

[0005] 2) The fermentation and cultivation period is long, generally 180-200 hours, resulting in high energy consumption

[0006] 3) Based on the above reasons, the cost of ivermectin fermentation is relatively high