An in vitro micro-dynamic culture device for mammalian embryos and a method for cultivating embryos therewith

Abstract

The invention discloses an in vitro micro-dynamic culture device of mammalian embryos and a method for cultivating embryos by using the same, relates to an in vitro culture device of embryos and a cultivation method using the same, and overcomes the defects of an in vitro oscillating micro-dynamic incubator of mammalian embryos and the defects of an existing patent technology. The culture device comprises a glass micro-fluid channel layer, a main body layer, an air chamber seal cover, an air duct, an injector, an electric motor and a square wave generator. The cultivation method comprises the following steps: 1, setting the frequency of the square wave generator and the stretching speed of the electric motor; 2, injecting a culture solution and mineral oil, and putting the embryos into a culture room; and 3, powering on a switch, and cultivating. The in vitro micro-dynamic culture device is applied to the field of biology.

Description

technical field [0001] The invention relates to an in vitro culture device for embryos and a culture method using the same. Background technique [0002] In order to simulate the internal environment of the reproductive system of female mammals, the current in vitro culture devices basically adopt swinging micro-dynamic culture. The oscillating micro-dynamic incubator is easy to cause the culture solution to spill, and such swaying and shaking will cause the embryos to continuously hit the petri dish wall of the hard petri dish, affecting the development of the embryo; moreover, the petri dish should be designed separately, and the bottom should be provided with square protrusions, In order to ensure that the culture dish will not fall or move during the cultivation process. [0003] In order to overcome the above-mentioned defects, the inventor invented the patent "a mammalian embryo in vitro culture device and its culture method" (patent number: ZL201210416329.7, applicat...

AI Technical Summary

Problems solved by technology

However, in the process of use, it is found that there are still many shortcomings in this patented technology: ①The magnetic sheet and the diaphragm are easy to separate and fall off, causing the incubator to fail to work normally; ②The electromagnetic radiation generated by the coil may cause abnormal or deformed embryonic development in the later stage. To a certain extent, it has adverse effects on the development of embryos; ③The magnetic sheet is directly in contact with the culture medium, and special materials such as NdFeB need to be selected, resulting in increased costs, and NdFeB is easy to powder and corrode, and the chemical treatment of the surface may also affect embryonic development

④The displacement of the diaphragm is fixed and cannot be adjusted, so it cannot meet the needs of different mammalian embryonic development

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