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Separation and purification method and identification method of chicken apolipoprotein AI

An apolipoprotein, separation and purification technology is applied in the field of separation, purification and identification of chicken apolipoprotein AI to achieve the effect of a simple separation and purification method

Inactive Publication Date: 2014-03-19
胡国良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no specific method for the separation, purification and identification of chicken apolipoprotein AⅠ

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] This specific embodiment takes the following technical scheme: the separation and purification method of its chicken apolipoprotein AI:

[0016] 1. Collection of chicken serum: Blood is collected from the subwing vein. After the blood is collected, remove the needle and gently inject it into the test tube. In order to prevent the rupture of blood cells, the cleaned and sterilized syringes and test tubes are infiltrated with 0.85% normal saline in advance to prevent blood clots from adhering to the glass tube wall and causing poor serum precipitation. Place the test tube in a 37°C incubator at a 45° angle for 1 hour, and then place it in a 4°C refrigerator for 3 to 4 hours. After the blood coagulates and clots shrink, use a capillary dropper to absorb the serum, then centrifuge at 1000×g for 10 minutes, and take the The clear serum was separated and stored in a -20°C refrigerator with NaN3 for later use.

[0017] 2. Preparation of serum high-density lipoprotein: take 1....

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PUM

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Abstract

The invention relates to a separation and purification method and an identification method of chicken apolipoprotein AI, and relates to the technical field of biology. The separation and purification method comprises the following steps: collecting chicken serums; preparing serum high-density lipoprotein; defatting HDL (High Density Lipoprotein); dissolving the defatted HDL; dialyzing and concentrating a defatted HDL solution; dialyzing the HDL solution by using a urea-10mmol / LTris-HCl-1mmol / LEDTA mixed solution of 6 mol / L to remove an organic solvent contained in the HDL solution, and then concentrating polyethylene glycol 6000 of 500 g / L to 2-3 mL to enhance the concentration of the HDL solution. The separation and purification method disclosed by the invention is simple and lays the foundation for the measurement and further research of the apo AI.

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to a method for separation, purification and identification of chicken apolipoprotein AI. Background technique: [0002] A large number of epidemiological studies have shown that serum high-density lipoprotein cholesterol (HDL-C) and apolipoprotein AⅠ (apoAI) are protective factors against arteriosclerosis, which are negatively correlated with atherosclerotic cardiovascular diseases. Although there is a very significant correlation between apoAI and HDL-C, with the deepening of research, many scholars believe that there are many differences in the physiological significance and clinical value of the two. Accounts for 70% of the HDL protein composition, reflecting the balance between HDL synthesis and breakdown. apoAI can directly act on the arterial wall to prevent the oxidation and aggregation of low-density lipoprotein (LDL), promote the outflow of cholesterol from the arterial wall...

Claims

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Application Information

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IPC IPC(8): C07K14/775C07K1/36C07K1/34C07K1/30G01N27/447
CPCC07K14/775
Inventor 胡国良
Owner 胡国良
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