A rapid chemiluminescent detection kit for quinolones and its testing method

Abstract

The invention relates to a rapid chemiluminiscence detection kit for quinolone drugs and a testing method of the kit. The kit is internally provided with a luminescent plate, a quinolones polyclonal antibody treated by a chemical primer, a goat-anti-rabbit antibody marked by horseradish peroxidase, a quinolones-series standard solution, a concentrated phosphate buffer, a concentrated washing solution and a luminescent solution, wherein the luminescent plate is provided with a coating antigen; the coating antigen is obtained by coupling a quinolone drugs dry hapten and OVA by adopting a mixed anhydride method; the quinolones polyclonal antibody treated by the chemical primer is obtained by taking a conjugate as an immunogen to immunize New Zealand white rabbits and the conjugate is prepared by coupling norfloxacin, an ofloxacin derivative and the OVA; the lowest detection limit of a chemiluminiscence enzyme-linked immunosorbent assay detection kit can be up to 0.1ng / ml, a linear detection range is 0.1ng / ml-8.1ng / ml, the in-plate variable coefficient is less than 20% and the recovery rate in a water sample ranges from 78% to 118%.

Description

technical field [0001] The invention relates to a direct chemiluminescence detection kit and a test method thereof, in particular to a rapid chemiluminescence detection kit and a test method for the residual amount of quinolones in samples such as animal tissues, eggs, and honey. Background technique [0002] Quinolones can be used to treat respiratory tract infection, genitourinary system infection, digestive system infection and other types of various infections, and can also be used for anti-tumor and anti-viral effects. In addition to being applied to the treatment of human diseases, it is also applied to the aquaculture industry. This type of drug is easy to induce bacterial resistance, so the management method for the use of food-producing animals is becoming more and more strict. Countries such as the European Union and North America only approve enrofloxacin as an antibacterial drug for animals, norfloxacin and ciprofloxacin Star has been banned for use in a...

AI Technical Summary

Problems solved by technology

[0007] 1) Heterogeneous reaction: In order to separate free substances and conjugated substances during the detection process, a multi-step plate washing process is required, which is time-consuming and laborious, and it is difficult to improve the automation of the operation;

[0008] 2) Enzyme-catalyzed reaction: use the enzyme-catalyzed substrate to develop color and measure the absorbance value of the reaction solution. The reaction time and temperature have a great influence on the enzyme activity, and the stability of the reagent is poor.

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