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Manioc waste anaerobic fermentation residue pretreatment and saccharification method

A technology of anaerobic fermentation and cassava residue, which is applied in the field of chemical engineering, can solve the problems of incomplete dissolution and uneven dissolution of inner cellulose, and achieve the effects of less three wastes, economical process, and good pretreatment effect.

Inactive Publication Date: 2014-03-12
CHANGZHOU UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

During the dissolution pretreatment process, part of the cellulose in contact with the ionic liquid dissolves quickly, thus forming a state in which the dissolved cellulose slurry wraps the undissolved cellulose, so that the inner layer of cellulose is not completely dissolved, so there is a dissolution Inhomogeneity

Method used

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  • Manioc waste anaerobic fermentation residue pretreatment and saccharification method

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Embodiment 1

[0022] The hydrochloric acid assisted [Mmim] DMP pretreatment cassava residue anaerobic fermentation residue of embodiment 1 mass final concentration 0.5%

[0023] In the pretreatment system, 2 g of dried cassava residues from anaerobic fermentation were treated with 90% (w / w) [Mmim]DMP, 0.5% (w / w) hydrochloric acid, and 9.5% (w / w) water mixture After dissolving and pretreatment at 130 °C for 15 min, deionized water was added to wash and regenerate to obtain regenerated cellulose raw materials. After the regenerated cellulose material was washed three times with deionized water, pH = 4.8 acetic acid-sodium acetate buffer (50 mM) was added to form a mixed system, the final mass concentration of the residual cellulose material was 5%, and then the final mass concentration was 0.3% Cellulase (Sigma, C-2730) was used for enzymatic hydrolysis, and the reaction was shaken on a constant temperature shaker at 50 ℃ and 180 rpm for 72 h, and the yield of reducing sugar was 40.8%.

Embodiment 2

[0024] The hydrochloric acid assisted [Mmim] DMP pretreatment cassava residue anaerobic fermentation residue of embodiment 2 mass final concentration 5%

[0025] In the pretreatment system, 2 g of dried cassava residues from anaerobic fermentation were treated with 75.5% (w / w) [Mmim]DMP, 5% (w / w) hydrochloric acid, and 19.5% (w / w) water mixture After dissolving and pretreatment at 110 °C for 90 min, add deionized water to wash and regenerate; after washing the regenerated cellulose material three times with deionized water, add pH=4.8 acetic acid-sodium acetate buffer (50 mM) to form a mixed system, and the residual fiber The final mass concentration of the raw material was 5%, and then the final mass concentration of 0.3% cellulase (Sigma, C-2730) was used for enzymatic hydrolysis, and the reaction was shaken on a constant temperature shaker at 50 °C and 180 rpm for 72 h, and the reducing sugar was produced. The rate is 42.6%. 2 g of dried cassava residues from anaerobic f...

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Abstract

The invention relates to a manioc waste anaerobic fermentation residue pretreatment and saccharification method, belonging to the technical field of microbes. The method comprises the following steps: in a 1,3-dimethylimidazole dimethylphosphate ([Mmim]DMP)-hydrochloric acid-water pretreatment system, carrying out dissolution pretreatment on manioc waste anaerobic fermentation residue at 110-130 DEG C for 15-90 minutes, washing with deionized water to regenerate a cellulose raw material; and washing the regenerated cellulose raw material three times, adding an acetic acid-sodium acetate buffer solution to form a mixed system, carrying out enzymolysis with cellulase, and carrying out oscillating reaction on a 50 DEG C constant-temperature oscillating table at 180 rpm, thereby achieving the goal of decomposing the manioc waste anaerobic fermentation residue. The dilute hydrochloric acid is used for assisting the ([Mmim]DMP) to pretreat the manioc waste anaerobic fermentation residue, thereby implementing high-value utilization of the manioc waste anaerobic fermentation residue; and the reducing sugar yield is higher than 40%. The method has the advantages of simple technique, high efficiency, economical process, fewer three wastes and the like.

Description

technical field [0001] The invention discloses a pretreatment of cassava residue anaerobic fermentation residue (hydrochloric acid-assisted 1,3-dimethylimidazole dimethyl phosphate [Mmim] DMP pretreatment) and a saccharification method, belonging to the field of chemical engineering. [0002] Background technique [0003] Cassava is one of the three largest potatoes in the world, with a global annual output of more than 130 million tons. The deep processing of cassava produces a large amount of cellulose-rich waste—cassava residue, which is usually used for animal feed or returned to the field, or even directly incinerated. These treatment methods not only waste lignocellulose resources, but also cause environmental pollution. Anaerobic fermentation of cassava residues to produce biogas (eg, methane) is an efficient way to utilize cassava residues. However, the residue formed still contains a large amount of lignocellulose. If not treated properly, the residue will still ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 何玉财龚磊王茜张跃王利群卿青陆婷吴殷琦吴昊张哲蓉
Owner CHANGZHOU UNIV
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