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A Surface-Enhanced Raman Spectroscopy Method for the Quantitative Detection of Polychlorinated Biphenyls

A surface-enhanced Raman and polychlorinated biphenyl technology, which is used in Raman scattering, test sample preparation, material excitation analysis, etc., to achieve good uniformity of morphology and particle size

Active Publication Date: 2016-08-17
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of SERS for quantitative measurement is still a difficult problem to be solved in practical applications

Method used

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  • A Surface-Enhanced Raman Spectroscopy Method for the Quantitative Detection of Polychlorinated Biphenyls
  • A Surface-Enhanced Raman Spectroscopy Method for the Quantitative Detection of Polychlorinated Biphenyls
  • A Surface-Enhanced Raman Spectroscopy Method for the Quantitative Detection of Polychlorinated Biphenyls

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Experimental program
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Embodiment 1

[0021] Substrate preparation and characterization of gold-coated silica materials

[0022] First, to synthesize 1-3nm gold seeds, add 0.5mL of 1.0M sodium hydroxide to 45mL of ultrapure water, followed by adding 50mM of THPC. Here, THPC acts as both a reducing agent and a surfactant. After the mixed solution was vigorously stirred for 5 min, 36 µL of 1.0 M chloroauric acid solution was added. The color of the reaction solution changed from colorless to dark brown immediately, the stirring speed was reduced, and the stirring was continued for 15 minutes. The obtained reaction solution was stored in a refrigerator at 4°C for future use. Then, APTMS-modified silica nanoparticles were synthesized. For the preparation of silica nanospheres, first mix 3mL of ammonia solution (30wt%) with 50mL of pure alcohol solution, then add 1.5mL of TEOS solution, and stir the mixed solution overnight at low speed. Take 10 mL milky white product and add excess APTMS (200 µL), and the mixture ...

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Abstract

The invention discloses a surface-enhanced Raman spectroscopy method capable of quantitatively detecting polychlorinated biphenyls, comprising the following steps: preparing uniform gold-coated silica nanoparticles, and using a coupling agent to fix the core-shell nanoparticles on Build a SERS substrate on a quartz glass sheet; connect the PCB aptamer to a thiol group at one end, and then covalently connect to the surface of the gold shell layer of the nanoparticle through the thiol group; soak the prepared SERS substrate in a solution containing the PCB, and wait for a sufficient time Take it out, wash it with deionized water, and then perform Raman spectroscopy measurement. The gold-coated silica nanoparticles prepared in the present invention have good morphology and particle size uniformity, and its plasmon resonance absorption occurs at 750nm, and at 785nm ‑1 There is a strong SERS enhancement effect under laser excitation.

Description

technical field [0001] The content of the present invention mainly includes the design and preparation of gold-coated silica core-shell nanoparticles connected with PCB aptamer single-stranded DNA, using the change characteristics of the surface-enhanced Raman scattering spectrum of its aptamer single-stranded DNA, Realize specific and quantitative measurement and analysis of PCB. Background technique [0002] Polychlorinated biphenyls (PCB: Polychlorinated biphenyls) are organic substances that are not easily decomposed in the environment. Most of them are toxic and will accumulate in organisms, causing environmental pollution and endangering the survival of animals and humans. For monitoring and quantitative analysis of PCBs, traditional methods include gas chromatography, liquid chromatography, liquid chromatography-mass spectrometry, and other methods. Spectral methods have also attracted people's attention, such as phosphorescence and fluorescence methods for PCB detec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N1/28
Inventor 黄青鲁逸林
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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