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Membrane filtration low-temperature sterilization process of lipopeptide surfactant fermentation material preparation liquid

The technology of surfactant and fermentation liquid is applied in the field of low-temperature sterilization process of lipopeptide surfactant fermentation ingredient liquid membrane filtration, which can solve the problem of high energy consumption for sterilization, save raw materials, improve labor productivity, and facilitate operation and management. Effect

Active Publication Date: 2014-03-05
BEIJING JIEBOTE ENERGY TECH
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to overcome the high energy consumption of fermentation liquid sterilization in small and medium-sized enterprises, especially the problems of sterilization of lipopeptide surfactant fermentation ingredients and recovery of fermentation waste liquid, the present invention provides a membrane filtration low-temperature sterilization process

Method used

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  • Membrane filtration low-temperature sterilization process of lipopeptide surfactant fermentation material preparation liquid

Examples

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example 1

[0012] Sugar and inorganic salts in the lipopeptide fermentation ingredient liquid except yeast extract are diluted through the ingredient tank (1), so that the components in the lipopeptide fermentation liquid formula reach the required concentration. The lipopeptide fermentation liquid formula is as follows: glucose 2%, NaNO 3 0.2%, NH 4 Cl0.3%, K 2 HPO 4 ·3H 2 O0.3%, NaH 2 PO 4 2H 2 O1%, MgSO 4 .7H 2 O0.02%, yeast extract 0.2%, MnCl 2 .4H 2 O2ppm. Stir to dissolve, put it into the coarse filter (3) through the circulation pump (2), control the pressure at 0.15-0.2 MPa, the primary filtrate flows into the heat exchanger (4) naturally, control the flow rate of the primary filtrate, and control the outflow temperature of the primary filtrate to 80°C, The heated primary filtrate naturally flows into the constant temperature tank (5), and the flow rate is controlled so that the residence time of the liquid in the constant temperature tank (5) is 30 minutes. The liquid ...

example 2

[0014] The lipopeptide fermentation broth is adjusted to pH 1-2 with sulfuric acid to crystallize the active ingredient lipopeptide, and let the lipopeptide settle to the bottom of the container. The upper layer is the supernatant containing no or a small amount of lipopeptide. Put the supernatant into the batching tank (1), adjust the pH to 7.5-8.0, and add an appropriate amount of carbon source and inorganic salt, so that the components in the lipopeptide fermentation broth reach the following content: 2% glucose, NaNO 3 0.2%, NH 4 Cl, K 2 HPO 4 ·3H 2 O0.3%, NaH 2 PO 4 .2H 2 O1%, MgSO 4 .7H 2 O0.02%, yeast extract 0.2%, MnCl 2 .4H 2 02ppm, adjust the pH again to 7.5-8.0, stir to dissolve, inject the coarse filter (3) through the circulating pump (2), control the pressure at 0.15-0.2 MPa, and the primary filtrate flows into the heat exchanger (4) naturally, and the primary filtrate is controlled Flow rate, control the primary filtrate outflow temperature of 80°C, th...

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Abstract

The present invention discloses a membrane filtration low-temperature sterilization process of a lipopeptide surfactant fermentation material preparation liquid. The process comprises that: raw materials excluding a yeast extract paste in a lipopeptide fermentation material preparation liquid are diluted through a material preparation tank (1) and then are pumped into a crude filter (3) through a circulation pump (2), wherein pressure is controlled to 0.15-0.2 MPa; the primary filtrate naturally flows into a heat exchanger (4), wherein a primary filtrate outflow temperature is controlled to 80 DEG C; the heated primary filtrate naturally flows into a constant temperature tank (5); the liquid being subjected to the constant temperature treatment passes through a circulation pump (6) and is pumped into a membrane filter (7); the filtrate being subjected to membrane filtration flows into a cooler (8), and the cooled filtrate directly enters a fermentation tank (10) through a pipeline; and the raw material yeast extract paste in the lipopeptide fermentation material preparation liquid is diluted 5 times, is subjected to sterilization with a high-temperature sterilization tank (9), is cooled to achieve a fermentation temperature of 35 DEG C, and then directly enters the fermentation tank (10). According to the present invention, recycling of the lipopeptide surfactant fermentation waste liquid is achieved, and zero sewage emission during the lipopeptide surfactant fermentation production process is achieved.

Description

technical field [0001] The invention relates to a low-temperature sterilization process of lipopeptide surfactant fermentation ingredient liquid membrane filtration. Background technique [0002] Sterilization of fermentation ingredients and materials is the most widely used process in industries such as biology, medicine, and food processing. Traditional industrial sterilization methods mainly include intermittent high-temperature sterilization and continuous high-temperature instant sterilization. But for small and medium-sized fermenting enterprises, especially the above two methods of small enterprises all have the disadvantages of high energy consumption and high operating costs. Lipopeptide is a new type of surfactant developed in recent years. It has a wide range of application values ​​in the fields of medicine, petrochemical industry, etc. The production of lipopeptide is a microbial fermentation method. The current fermentation concentration is 0.5-2.4g / L. There ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/02A61L2/04
Inventor 曲红涛陈亮
Owner BEIJING JIEBOTE ENERGY TECH
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