A low-temperature sterilization process of lipopeptide surfactant fermentation ingredients liquid membrane filtration

A surfactant and fermentation liquid technology, applied in the field of lipopeptide surfactant fermentation ingredient liquid membrane filtration low-temperature sterilization process, can solve the problem of high energy consumption for sterilization, save raw materials, improve labor productivity, and facilitate operation and management Effect

Active Publication Date: 2017-07-28
BEIJING JIEBOTE ENERGY TECH
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Problems solved by technology

[0003] In order to overcome the high energy consumption of fermentation liquid sterilization in small and medium-sized enterprises, especially the problems of sterilization of lipopeptide surfactant fermentation ingredients and recovery of fermentation waste liquid, the present invention provides a membrane filtration low-temperature sterilization process

Method used

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  • A low-temperature sterilization process of lipopeptide surfactant fermentation ingredients liquid membrane filtration

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example 1

[0012] Sugar and inorganic salts in the lipopeptide fermentation ingredient liquid except yeast extract are diluted through the ingredient tank (1), so that the components in the lipopeptide fermentation liquid formula reach the required concentration. The lipopeptide fermentation liquid formula is as follows: glucose 2%, NaNO 3 0.2%, NH 4 Cl 0.3%, K 2 HPO 4 ·3H 2 O 0.3%, NaH 2 PO 4 2H 2 O 1%, MgSO 4 ·7H 2 O0.02%, yeast extract 0.2%, MnCl 2 4H 2 O2ppm. Stir to dissolve, put into the coarse filter (3) through the first circulating pump (2), control the pressure of 0.15 ~ 0.2 MPa, the primary filtrate flows into the heat exchanger (4) naturally, control the flow rate of the primary filtrate, and control the outflow temperature of the primary filtrate to 80 ℃, the heated primary filtrate naturally flows into the constant temperature tank (5), the flow rate is controlled so that the residence time of the liquid in the constant temperature tank (5) is 30min, and the liqui...

example 2

[0014] The lipopeptide fermentation broth is adjusted to pH 1-2 with sulfuric acid to crystallize the active ingredient lipopeptide, and let the lipopeptide settle to the bottom of the container. The upper layer is the supernatant containing no or a small amount of lipopeptide. Put the supernatant into the batching tank (1), adjust the pH to 7.5-8.0, and add an appropriate amount of carbon source and inorganic salt, so that the components in the lipopeptide fermentation broth reach the following content: 2% glucose, NaNO 3 0.2%, NH 4 Cl, K 2 HPO 4 ·3H 2 O 0.3%, NaH 2 PO 4 2H 2 O 1%, MgSO 4 ·7H 2 O0.02%, yeast extract 0.2%, MnCl 2 4H 2 O 2ppm, adjust pH 7.5~8.0 again, stir to dissolve, squeeze into the coarse filter (3) through the first circulating pump (2), control the pressure 0.15~0.2 MPa, the first filtrate flows into the heat exchanger (4) naturally, Control the flow rate of the primary filtrate, control the outflow temperature of the primary filtrate to 80°C, t...

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Abstract

The invention discloses a lipopeptide fermentation ingredient liquid membrane filtration low-temperature sterilization process. Raw materials in the lipopeptide fermentation ingredient liquid except yeast paste are diluted through an ingredient tank (1) and pumped into crude oil by a circulation pump (2). Filter (3), control pressure 0.15 ~ 0.2 MPa, primary filtrate flows into heat exchanger (4) naturally, controls primary filtrate outflow temperature 80 ℃, heated primary filtrate naturally flows into constant temperature tank (5), the liquid after constant temperature Squeeze into the membrane filter (7) through the circulation pump (6), the filtrate after the membrane filtration flows into the cooler (8), and the membrane filtrate after cooling directly enters the fermenter (10) through the pipeline; the raw material in the lipopeptide fermentation ingredient liquid After the yeast extract is diluted 5 times, it is sterilized by a high-temperature sterilization tank (9), cooled to a fermentation temperature of 35° C., and directly enters a fermenter (10). The invention realizes the recovery and utilization of the lipopeptide surfactant fermentation waste liquid, and realizes zero sewage discharge in the lipopeptide surfactant fermentation production process.

Description

technical field [0001] The invention relates to a low-temperature sterilization process of lipopeptide surfactant fermentation ingredient liquid membrane filtration. Background technique [0002] Sterilization of fermentation ingredients and materials is the most widely used process in industries such as biology, medicine, and food processing. Traditional industrial sterilization methods mainly include intermittent high-temperature sterilization and continuous high-temperature instant sterilization. But for small and medium-sized fermenting enterprises, especially the above two methods of small enterprises all have the disadvantages of high energy consumption and high operating costs. Lipopeptide is a new type of surfactant developed in recent years. It has a wide range of application values ​​in the fields of medicine, petrochemical industry, etc. The production of lipopeptide is a microbial fermentation method. The current fermentation concentration is 0.5-2.4g / L. There ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/02A61L2/04
Inventor 曲红涛陈亮
Owner BEIJING JIEBOTE ENERGY TECH
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