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Panax japonicus saponin as well as preparation method and application thereof

A technology of bamboo ginseng saponin and bamboo ginseng, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, antibody medical ingredients, etc., and can solve problems such as crystallization precipitation, inability to freeze-dry, and colloidal state destruction

Active Publication Date: 2014-03-05
湖北森元药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous emergence of new vaccines, aluminum salt adjuvants have shown some problems, including: (1) Due to their physical and chemical properties, the colloidal state of aluminum salt vaccines is destroyed after freezing, so they cannot be freeze-dried and transported at low temperatures, But many peptide vaccines now must be stored at low temperature
(2) Due to the high salt content, there will be crystal precipitation after long-term storage, so that the stability of the prepared vaccine cannot be guaranteed
(6) Aluminum salt adjuvant releases antigenic substances slowly, and the effect is relatively weak. It is not effective enough for many soluble antigens, and the requirements for antigens are relatively high.
The lethal dose of soap shake QS-21 to mice is 500 μg, although the toxicity is less than that of Quil A, it is still only used in tumor vaccines
Moreover, there is a cool bond structure in the molecular structure of saponin QS-21, which affects its stability.

Method used

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  • Panax japonicus saponin as well as preparation method and application thereof
  • Panax japonicus saponin as well as preparation method and application thereof
  • Panax japonicus saponin as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Embodiment 1: Preparation of alcohol-precipitated bamboo ginseng saponin of the present invention

[0110] The implementation steps of this embodiment are as follows:

[0111] A. Bamboo Ginseng Extraction

[0112] Crush the raw material of bamboo ginseng, according to the weight ratio of bamboo joints to ethanol aqueous solution 1:5, soak the crushed bamboo ginseng in 55% ethanol aqueous solution by weight, soak for 2 hours, and then heat and reflux extraction for 3 times, 1 hour each time , combined extracts, and concentrated under reduced pressure at a pressure of 0.006MPa to obtain a concentrated solution;

[0113] B. Extraction and separation

[0114] The concentrated solution obtained in step A is extracted multiple times with n-butanol and separated from the two phases according to the volume ratio of the concentrated solution and n-butanol at 1:1 until the n-butanol phase is colorless, and the obtained n-butanol extracts are combined, and then Concentrate unde...

Embodiment 2

[0124] Example 2: Preparation of alcohol-precipitated bamboo ginseng saponin of the present invention

[0125] The implementation steps of this embodiment are as follows:

[0126] A. Bamboo Ginseng Extraction

[0127] Crush the raw material of bamboo ginseng, according to the weight ratio of bamboo joints to ethanol aqueous solution 1:15, soak the crushed bamboo ginseng in 85% ethanol aqueous solution by weight, soak for 5h, and then heat and reflux for extraction twice, each time for 5h , combined extracts, and concentrated under reduced pressure at a pressure of 0.008MPa to obtain a concentrated solution;

[0128] B. Extraction and separation

[0129] The concentrated solution obtained in step A is extracted multiple times with n-butanol and separated from the two phases according to the volume ratio of the concentrated solution and n-butanol at 1:4 until the n-butanol phase is colorless, and the obtained n-butanol extracts are combined, and then Concentrate under reduced...

Embodiment 3

[0133] Example 3: Preparation of alcohol-precipitated bamboo ginseng saponin of the present invention

[0134] The implementation steps of this embodiment are as follows:

[0135] A. Bamboo Ginseng Extraction

[0136] Crush the bamboo ginseng raw material, according to the weight ratio of the bamboo joints to the ethanol aqueous solution 1:6, soak the crushed bamboo ginseng in an ethanol aqueous solution with a concentration of 60% by weight, soak for 2.6h, and then heat and reflux for extraction 3 times, each time 1.8h, the extracts were combined, and then concentrated under reduced pressure at a pressure of 0.001MPa to obtain a concentrated solution;

[0137] B. Extraction and separation

[0138]The concentrated solution obtained in step A is extracted multiple times with n-butanol and separated from the two phases according to the volume ratio of the concentrated solution to n-butanol 1:1.8 until the n-butanol phase is colorless, and the obtained n-butanol extracts are co...

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Abstract

The invention relates to panax japonicus saponin used as a vaccine adjuvant as well as a preparation method and application thereof. The method comprises the following steps: extraction of the panax japonicus, extraction separation, resin adsorption and the like. The panax japonicus saponin is further separated and purified, the weaknesses of the traditional method that only the total saponins component is used and the main components of the samponin is not studied can be overcome, so that the problem that the drug ingredients are not known by people can be solved; an active part of the panax japonicus saponin has an obvious immunity adjuvant effect, and a way for developing the vaccine adjuvant is provided.

Description

【Technical field】 [0001] The invention belongs to the technical field of vaccine adjuvants. More specifically, the present invention relates to a bamboo ginseng saponin used as a vaccine adjuvant, a preparation method of the bamboo ginseng saponin, and a use of the bamboo ginseng saponin. 【Background technique】 [0002] Bamboo ginseng is a plant of the genus Panax ginseng in the Araliaceae family. The dried rhizome of Bamboo ginseng is in the shape of a bamboo whip. It is one of the rare and commonly used Chinese medicinal materials in China. Bamboo ginseng mostly grows in broad-leaved sparse forest shady wetlands or beside rocks and ditches above an altitude of 1000m. It has high requirements on soil conditions and is suitable for growth in neutral or slightly acidic environments. It likes thick humus layers, loose and fertile, and drainage Good sandy loam. It has a long history of folk application in Yunnan, Guizhou, Sichuan, Hubei, Shaanxi, Gansu, Henan, Zhejiang, Anhui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P37/04
Inventor 袁丁张长城刘朝奇王婷孙志伟周志勇崔倩倩万静枝何毓敏
Owner 湖北森元药业有限公司
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