Breeding method of a blue-shank recessive white-feathered chicken strain
A technology of recessive white feather chicken and green shank, which is applied in animal husbandry and other fields, can solve the problems of slow growth, poor meat quality, and low feed conversion rate, and achieve the effect of tender meat, avoiding tediousness, and delicious meat
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[0033] In the present embodiment, the breeding method of the blue-shank recessive white-feathered chicken strain may further comprise the steps:
[0034] (1) Using the Qingshan recessive white feather chicken as the male parent and the white Laihang chicken as the female parent in the Gushi chicken population to perform crossbreeding to produce the F1 generation;
[0035] (2) The F1 generation is self-crossed to obtain the F2 generation, and the F2 generation of green shin and white feather male and female chickens are selected;
[0036] (3) Determination of the genotype of the F2 generation blue-shin and white-feather male and female chickens by AS-PCR method, specifically including the following steps:
[0037] ① Collection of samples
[0038] Take blood from the veins of the chicken wings with green shanks and white feathers selected from the F2 generation, add 1 / 3 anticoagulant, extract blood DNA with proteinase K method, and store the DNA samples in a refrigerator at 4°C...
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