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Primer, kit and detection method for detecting EGFR (Epidermal Growth Factor Receptor) gene hotspot mutation

A kit and gene technology, applied in the field of molecular biology, can solve the problems of high equipment requirements, high data analysis requirements, and high requirements, and achieve the effect of simple and fast detection process, high detection sensitivity and low price

Inactive Publication Date: 2014-02-26
北京海思特医学检验实验室有限公司
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  • Application Information

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Problems solved by technology

This method has high requirements for paraffin-embedded tissue specimens, requiring the tumor tissue content in the specimens to be more than 20%, low detection sensitivity, complex and cumbersome detection process, high requirements for data analysis, and there is no commercial kit at present, which is difficult for laboratory instruments. Equipment requirements are also higher

Method used

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  • Primer, kit and detection method for detecting EGFR (Epidermal Growth Factor Receptor) gene hotspot mutation
  • Primer, kit and detection method for detecting EGFR (Epidermal Growth Factor Receptor) gene hotspot mutation
  • Primer, kit and detection method for detecting EGFR (Epidermal Growth Factor Receptor) gene hotspot mutation

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Embodiment

[0039] Kit for detecting EGFR gene hot spot mutation and its use

[0040] 1. The kit for detecting EGFR gene hot spot mutation contains:

[0041] (1) Primers

[0042] Two pairs of EGFR gene EXON19 deletion mutation-specific forward and reverse primers, wherein the first pair of EGFR gene EXON19 deletion mutation-specific forward and reverse primers are shown in SEQ ID NO: 1 and SEQ ID NO: 2, and the first pair of EGFR gene EXON19 deletion mutation-specific forward and reverse primers Two pairs of EGFR gene EXON19 deletion mutation-specific forward and reverse primers are shown in SEQ ID NO: 1 and SEQ ID NO: 3;

[0043] Two pairs of EGFR gene EXON20T790M specific forward and reverse primers, wherein, the first pair of EGFR gene EXON20T790M specific forward and reverse primers are as shown in SEQ ID NO:4 and SEQ ID NO:5, the second pair of EGFR gene The forward and reverse primers specific to EXON20T790M are shown in SEQ ID NO:6 and SEQ ID NO:5;

[0044] Two pairs of forward ...

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Abstract

The invention discloses a primer, a kit and a detection method for detecting EGFR (Epidermal Growth Factor Receptor) gene hotspot mutation. The sequence of the primer is shown as SEQ ID NO:1-SEQ ID NO:9, the kit comprises the primer, PCR (Polymerase Chain Reaction) MIX reaction liquid, restriction endonuclease for enzyme digestion aiming at different mutations, BAS (Boric Acid Solution), enzyme digestion buffer solution, a negative control substance, a positive control substance, deionized water, bottles or tubes or packaging boxes for isolating or centrally packaging reagents. According to the primer, the kit and the detection method for detecting EGFR gene hotspot mutation, the three most common hotspot mutations of the EGFR genes can be fast detected by taking peripheral blood as a sample, the detection sensitivity is high, the time consumption is less, and the clinically needed data can be obtained in first time.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a gene mutation detection primer and a kit, in particular to a primer, a kit and a detection method for detecting EGFR gene hot spot mutation. Background technique [0002] Epidermal growth factor receptor (EGFR) is a transmembrane receptor tyrosine kinase, which is a member of the receptor tyrosine kinase (TK) erbB / HER family. Phosphorylation plays an important role in regulating the growth, invasion, transformation, survival, angiogenesis and metastasis of tumor cells. Studies have shown that EGFR is overexpressed in many malignant tumors. [0003] In recent years, with the development of medical molecular biology, molecular targeted therapy, as a new means of malignant tumor treatment, has been paid more and more attention by clinicians. Therefore, EGFR has received widespread attention as a molecular target for malignant tumor treatment. Epidermal growth factor recep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/106C12Q2600/156
Inventor 梁超熊凯程宇邢军英刘晓峰侯然朱立文王绪华方国伟黄士昂陈忠
Owner 北京海思特医学检验实验室有限公司
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