Method of preparing functional monascus mycelia and fermentation liquor by liquid fermentation, and products of the monascus mycelia and the fermentation liquor
A technology for functional red yeast rice and red yeast yeast mycelium, which is applied in the field of red yeast yeast fermentation broth, can solve the problems of product hygiene standards being difficult to meet international standards, biomass testing is difficult, and it is not suitable for large-scale production. The effect of utilization rate and product yield, high quality stability and short cycle time
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Embodiment 1
[0031] A mutagenic strain Monascus pilosus Zheng YZ101, its biological deposit number is: CGMCC8167, was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on September 16, 2013, and the address of the center is: Beijing No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Institute of Microbiology, Chinese Academy of Sciences; the classification name is Monascus pilosus.
[0032] In other embodiments, other Monascus strains bred by mutagenesis can also be selected, such as Monascus purpureus YangYZ202, Monascusanka YangYZ201, Monascus ruber YangYZ102 and other strains.
[0033] A method for preparing functional red yeast mycelium and red yeast fermentation liquid by liquid fermentation of aforementioned mutagenic strains, comprising the following steps:
[0034] (1) preparation of fermentation medium: cereal starch raw material is mixed with fermentation medium;
[0035] (2) Preparation of strains: preparation of mutagen...
Embodiment 2
[0052] The red koji mutagenic strain provided in this example, the method and products for producing functional red koji mycelia and red koji fermentation liquid by liquid fermentation using the mutagenic strain and cereal raw materials are basically the same as in Example 1, and the difference lies in:
[0053] The method for preparing functional red yeast mycelium and red yeast fermentation liquid by liquid fermentation of aforementioned mutagenic strains comprises the steps of:
[0054] (1) preparing Monascuspilosus ZhengYZ101 as a fermentation strain;
[0055] (2) Preparation of production medium:
[0056] a, the preparation of slant medium: get wort juice 10Be as universal medium, boil, pack into test tube by 40% by weight respectively, sterilize, cool, make slant medium, insert and the purple that prepares in step 1 Monascus strains were cultured on slant medium.
[0057] B, the preparation of special culture medium for slant: get 8% soluble starch calculated accordi...
Embodiment 3
[0066] The red yeast mutagenesis strain provided in this example, the methods and products for producing functional red yeast mycelium and red yeast fermentation liquid by liquid fermentation using the mutagenic strain and cereal raw materials are basically the same as those in Examples 1 and 2. , the difference being:
[0067] The method for preparing functional red yeast mycelium and red yeast fermentation liquid by liquid fermentation of aforementioned mutagenic strains comprises the steps of:
[0068] (2) Preparation of production medium:
[0069] a, the cultivation of slant medium: get wort juice 15Be as general medium, boil, make slant medium nutrient solution, pack eggplant bottle by the amount of 45% by weight respectively, sterilize, cool down, insert and step 1 The Monascus red bacteria prepared in the slant medium were cultured.
[0070] B, the cultivation of inclined-plane special medium: get 10% soluble starch calculated according to weight percentage, maltose 5...
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