A salt-tolerant denitrification compound bacterial agent and its preparation and use method

Abstract

The invention belongs to the field of biotechnology, and in particular relates to a salt-tolerant and denitrification compound bacterial agent and its preparation and application method. Salt-resistant denitrification compound bacterial agent consists of Halomonas with denitrification characteristics and nitrous nitrogen accumulation in the denitrification process, Halomonas with denitrification characteristics and no nitrite nitrogen accumulation in the denitrification process, and Nitrification The specific Bacillus and Halomonas with salt-tolerant characteristics are mixed according to the volume ratio of 1:1:(10~20):(60~85). Nitrogen treatment, the denitrification rate is at least 95%, and the effluent water quality meets the standard discharge. In the composite bacterial agent of the present invention, the four kinds of microorganisms interact and influence each other, and the removal rate of ammonia nitrogen and total nitrogen is higher than that of any microorganism used alone. Because the impact resistance of the composite bacterial agent is stronger than that of purified bacterial strains, it can effectively Promote simultaneous nitrification and denitrification, so that the denitrification effect of high-salt wastewater is significantly improved.

Description

Technical field [0001] The invention belongs to the field of biological technology, and specifically relates to a salt-tolerant denitrification composite bacterial agent and a preparation and use method thereof. Background technique [0002] In addition to a large amount of organic pollutants, high-salt wastewater also has a large amount of chloride ions, which has a negative impact on the traditional biochemical treatment of high-salt wastewater. Inorganic salts play an important role in promoting enzyme reaction, maintaining membrane balance and regulating osmotic pressure during the growth of microorganisms. However, if the chloride ion concentration is too high, it will inhibit the growth of microorganisms. The main reasons are: [0003] (1) When the salt concentration is too high, the osmotic pressure will be high, which will dehydrate the microbial cells and cause the cell protoplasm to separate; [0004] (2) In the case of high salt concentration, the salting-out effect will...

AI Technical Summary

Problems solved by technology

[0004] (2) In the case of high salt concentration, the salting-out effect will reduce the activity of dehydrogenase;

[0005] (3) High chloride ion concentration is toxic to bacteria;

[0006] (4) Due to the increase of water density, activated sludge is easy to float and lose

At present, the treatment of high-salt wastewater can be divided into two categories: physical chemical method and biological method. Due to the high content of dissolved organic matter in this type of wastewater, general physical and chemical methods are difficult to deal with and the treatment cost is high. Biological treatment method is economical, Efficient and harmless, it is still the preferred treatment method

However, when the wastewater contains temporary toxic substances, which are toxic to microorganisms and treated with general biological methods, the degradation rate is slow, and the bacteria need a long period of time to adapt, and the target pollution can be quickly and effectively degraded by adding dominant strains thing

Since the biological treatment system is a very complex system, the best treatment state cannot be achieved by adding a single strain