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Method for detecting mitoxantrone based on luminous gold nanocluster

A technology of mitoxantrone and gold nanoclusters is applied in the field of mitoxantrone drug detection to achieve the effects of high sensitivity, rapid detection and high selectivity

Active Publication Date: 2014-02-12
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a new method for detecting the anticancer drug mitoxantrone based on water-soluble luminescent gold nano-cluster biosensing fluorescence detection method, so as to solve the deficiencies in the existing detection technology of mitoxantrone

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  • Method for detecting mitoxantrone based on luminous gold nanocluster
  • Method for detecting mitoxantrone based on luminous gold nanocluster
  • Method for detecting mitoxantrone based on luminous gold nanocluster

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Embodiment 1

[0015] Synthesis of fluorescent nano-gold clusters using BSA as a template: The synthesis of fluorescent nano-gold clusters is based on the method reported in the literature [see: Xie J., Zheng Y., Ying J. Y. J. Am. Chem. Soc. , 2009, 131, 888-889.], made some improvements, the process is as follows: First, 1g of tetrachloroauric acid was dissolved in 100 mL of double distilled water to form a 10 mg / mL stock solution. Then, take 4.1 mL of the newly prepared solution and add it to 10 mL of the 50 mg / mL bovine serum albumin solution at a reaction temperature of 37 ? C. During the period, stir vigorously to make it evenly mixed. After 2 minutes, add 1 mL of 1.0 mol / L NaOH solution, continue to stir for 12 hours, keep the temperature at 37 ? C. After the improvement, the synthesis time was shortened from three days to 12 hours. The prepared gold nanocluster probe has stable fluorescence characteristics, with a maximum emission peak at a wavelength of 619 nm and an excitation wavel...

Embodiment 2

[0022] Use the prepared fluorescent gold nanocluster probe to detect the anticancer drug daunorubicin. Specific steps: Take 300 μL of the prepared gold nanocluster sample, add it to a centrifuge tube, and then add a series of concentrations of daunorubicin drug working solution , To detect the effects of different concentrations of daunorubicin on the fluorescence signal of gold nanocluster probes (final concentrations are 0, 2, 4, 10, 20, 50, 70, 100, 110, 120, 130, 140 μg / mL ). Dilute with double distilled water to a final volume of 3 mL, mix well, and measure. Under the excitation of 469 nm wavelength light, the fluorescence spectrum of gold nanoclusters was tested, the slit width of the instrument was set to 5 nm, and the fluorescence intensity of the emission peak at the wavelength of 619 nm was detected. Test the fluorescence signal of the sample after adding the drug, and record the fluorescence intensity at the position of the maximum emission peak, expressed as: Δ F ...

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Abstract

The invention discloses a method for detecting mitoxantrone based on a luminous gold nanocluster. The method comprises the following steps: preparing the gold nanocluster, measuring fluorescence intensity of the gold nanocluster at a position with wavelength of 619 nm and recording as F0 under excitation of 469nm-wavelength light; after adding mitoxantrone with different weight in the step I, measuring the fluorescence intensity of the gold nanocluster containing the mitoxantrone with different concentrations and recording as F at the position with wavelength of 619 nm under excitation of 469nm-wavelength light, calculating out linear relation between change value of the fluorescence intensity of the gold nanocluster before and after adding the mitoxantrone and mitoxantrone concentration; adding a to-be-detected sample into the gold nanocluster, and testing the change value of the fluorescence intensity at the position with wavelength of 619 nm under excitation of 469nm-wavelength light before and after adding the mitoxantrone, calculating the weight or concentration of the added mitoxantrone to establish detection according to the obtained linear relation. The method has characteristics of being high in selectivity, high in sensitivity, simple and convenient, easy to implement, and the like.

Description

technical field [0001] The invention belongs to the field of mitoxantrone drug detection, in particular to a novel method for detecting anticancer drug mitoxantrone based on biosensing fluorescence of water-soluble luminescent nano-gold clusters. Background technique [0002] Mitoxantrone is a new type of anticancer drug that is widely used clinically. It belongs to the anthraquinone class of anticancer drugs. It has significant clinical effects on most malignant tumors [see: Thackery, Ellen. The Gale Encyclopedia of Cancer : L-Z. Detroit: Thomson Gale. 2002, pp: 708-710.], the quantitative determination of the drug is helpful for clinical medical diagnosis. At present, the methods for the determination of anthraquinone anticancer drugs mainly include high performance liquid chromatography, capillary electrophoresis, and HPLC-MS, but there are few studies on spectral analysis, and the only fluorescence spectroscopy and spectrophotometry are mostly flawed. For example, the ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 冯大千刘国良王伟
Owner YANCHENG INST OF TECH
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