Preparation method of tripterine

Abstract

The invention provides a preparation method of tripterine. The method is characterized by comprising the particular steps that fresh celastrus orbiculatus thunb roots are cleaned, sliced, dried and smashed into celastrus orbiculatus thunb powder; the celastrus orbiculatus thunb powder is soaked and extracted; a solvent is recovered by an extracting solution; an extractive extract is obtained; the extractive extract is dissolved by an organic solvent; a silicagel column is arranged by a dry method; methylene dichloride and methanol gradient elution is performed; an eluation part containing the tripterine is collected; the solvent is recovered; crude tripterine is obtained; the crude tripterine is dissolved by the organic solvent; the silicagel column is arranged by the dry method; the methylene dichloride and methanol gradient elution is performed; the eluation part containing the tripterine is collected; the solvent is recovered; pure tripterine is obtained. The method is simple to operate, simple and convenient in technology, low in instrument and equipment requirement, and high in product yield; an extractive obtained by a soaking method is directly subjected to twice silicagel column layer separation, so that means such as solvent extraction and acid and alkali treatment can be avoided; the soaked solvent can be repeatedly used after recovery; the method is suitable for industrial production.

Description

technical field [0001] The present invention relates to a new method for preparing tripteryglobulin from S. chinensis, in particular to a preparation method for extracting, separating and purifying tripterygne from S. sagera, which belongs to the technology of natural active product preparation method field. Background technique [0002] Celastrus orbiculatus Thunb is the root of Celastrus orbiculatus Thunb. This product has the effects of expelling wind and dehumidification, promoting blood circulation and detoxification, and reducing swelling. Indications for muscle and bone pain, numbness of limbs, rheumatoid arthritis, lumbago and leg pain, amenorrhea, convulsions in children, diarrhoea, dysentery, swollen poison, snake bites, bruises. Folks often use this medicine in decoction or external application to treat rheumatic arthralgia. The decoction and tincture of this medicine have been clinically used to treat rheumatoid arthritis for many years, and the curative effect...

AI Technical Summary

Problems solved by technology

Tripterygium is a triterpenoid with a complex structure and low content in plants. The separation and purification process is complicated, which brings difficulties to the preparation of tripterine, and it is often impossible to obtain pure products

There are many ways to prepare red pigment, some use ethanol reflux extraction, silica gel or alumina column separation, some use ultrasonic extraction, the extract is precipitated with acid and alkali, alumina column chromatography and other processes, in the process of carrying out the above During the experiment, they all said that they could obtain pure products, and some even applied for patents. However, it is still difficult for their experimental methods to obtain pure products with high purity.

[0007] As can be seen from the above, the existing preparation process steps are relatively long, the operation is cumbersome, and the product yield is not high. The key is that most of them cannot obtain pure products in the true sense.

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