Preparation method and application of swine vaccine specific cattle spleen transfer factor (TF)

Abstract

The invention discloses a preparation method of a swine vaccine specific cattle spleen transfer factor (TF). The preparation method comprises the following steps: slaughtering a cattle with a positive swine vaccine antibody to harvest the cattle spleen; preserving the cattle spleen at low temperature; unfreezing the cattle spleen; removing fasciae; mincing the cattle spleen; homogenizing the pulp; filling the homogenized pulp into a bottle and storing the pulp; repeatedly freezing and thawing the pulp; centrifuging the pulp; carrying out microfiltration; carrying out ultrafiltration; carrying out inactivation; regulating the pH value; regulating the osmotic pressure; removing bacteria; and detecting the quality. The preparation method has the advantages that the pH value of water for injection is regulated to 4-6 in the pulp homogenizing step, thus being beneficial to increasing of the yields of ribose and polypeptide; a box type membrane coating is adopted for tangential flow filtration, so that the dead volumes of system residues are small and linear amplification production is easy to achieve; a 1-3KD box type membrane coating is adopted to carry out tangential flow nanofiltration on a TF crude product, thus preparing the high-concentration TF and improving the using effects; phenol red is used as an acid-base indicator, thus being convenient for clients to observe the pH value of the TF; the TF uses beta-propiolactone for inactivation instead of traditional formaldehyde and an osmotic pressure regulation process is added, thus being beneficial to combined immunization of the TF and vaccines.

Description

technical field [0001] The invention belongs to transfer factor, and in particular relates to a preparation method and application of porcine vaccine-specific bovine spleen transfer factor. Background technique [0002] Transfer Factor (TF) is a substance released by T lymphocytes that can transfer sensitization information. It can specifically transfer the donor's cellular immune information to the recipient, thereby enhancing the immune function of the recipient. Since the discovery of TF in the 1950s, scholars at home and abroad have conducted a lot of research on it, and introduced it into veterinary immunity and clinical treatment in 1979. At present, TF has gradually become a widely used immune enhancer. [0003] TF has no species specificity, so TF has a wide range of sources. Human TF, pig TF, cattle TF, sheep TF, goose TF, monkey TF, etc. are common in human clinical practice, while bovine TF and pig TF are common in livestock and poultry breeding. Chicken TF. [...

AI Technical Summary

Problems solved by technology

Infection of suckling piglets within 7 days of age is the most common. In severe cases, it can lead to the disease of the whole group, resulting in the death of a large number of suckling piglets. The weight gain of nursery pigs and fattening pigs slows down, causing great losses to the pig industry.

The Lawrence method is routinely used, that is, the transfer factor is recovered by two dialysis methods. This method is simple to operate, but the production cycle is long, time-consuming, wasteful, ineffective and labor-intensive.

In addition, even if the tangential flow filtration method is adopted, hollow fiber membranes are mostly used for tangential flow filtration. The hollow fiber membranes are difficult to clean, and the entire component needs to be replaced after the membrane is damaged. The residual dead volume of the system is large, and the flow resistance in the fiber tube is very large. , Large pressure loss, not suitable for handling viscous liquids, not easy to linearly scale up production

These methods are more difficult to scale up production to meet the needs of the market

[0021] In the original method of preparing TF, due to the selection of formaldehyde inactivation and the lack of osmotic pressure adjustment process in the inactivation process, the prepared TF is not suitable for use as a diluent and immune adjuvant for porcine vaccines, that is, it cannot directly dilute porcine attenuated vaccines, and cannot be used with Porcine attenuated vaccine virus solution mixed and freeze-dried

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