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System for quantitative chemical analysis of samples, in particular in the medical field, with calibration of the instrumental response, and the corresponding method

An analysis system and quantitative analysis technology, applied in the field of analysis, can solve the problems of expensive isotope standards, repetition, etc., and achieve the effect of cost saving

Active Publication Date: 2014-02-05
马泰奥·弗洛里迪亚 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0035] The main disadvantage of the second method and approach is that it requires the use of extremely expensive isotope standards, which limits the practical use of the method to centers that regularly process large numbers of samples, such as hospitals, forensic analysis centers, quality control and similar experiments room
[0041] It is worth noting that the calibration of the instrument used for the analysis must be repeated each time; in other words, it must be performed before analyzing each sample, which, as already stated, constitutes an important drawback of the prior art

Method used

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  • System for quantitative chemical analysis of samples, in particular in the medical field, with calibration of the instrumental response, and the corresponding method
  • System for quantitative chemical analysis of samples, in particular in the medical field, with calibration of the instrumental response, and the corresponding method
  • System for quantitative chemical analysis of samples, in particular in the medical field, with calibration of the instrumental response, and the corresponding method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0196] Example 1 - Analysis of cocaine and its metabolites

[0197] In this example, the PROSAD technique was used to quantify cocaine and its metabolite benzoylecgonite in urine samples.

[0198] A binary chromatographic gradient was used comprising phase A) H2O + 0.05% formic acid and phase B) CH3CN + 0.05 formic acid. Given the time T=0 at the time of injection, %B was 15%. Maintain this condition for 2 minutes. After this interval, %B increased to a value of 70% within 8 minutes. Initial conditions were restored over the next two minutes. The instrument acquisition time was set at 24 minutes. A ThermolEctron C8 150×1 mm chromatographic column was used. The surface potential, electrospray potential and surface temperature were 50V, 0V and 110°C, respectively. The nebulisation gas flow rate was 2 L / min.

example 2

[0199] Example 2 - Testosterone analysis in plasma samples

[0200] The PROSAD system was used to analyze testosterone in human plasma.

[0201] A binary chromatographic gradient was used comprising phase A) H2O + 0.05% formic acid and phase B) CH3CN + 0.05 formic acid. Given the time T=0 at the time of injection, %B was 15%. Maintain this condition for 2 minutes. After this interval, %B increased to a value of 70% within 8 minutes. Initial conditions were restored over the next two minutes. The instrument acquisition time was set at 24 minutes. A ThermolEctron C8 150×1 mm chromatographic column was used. The surface potential, electrospray potential and surface temperature were 50V, 0V and 110°C, respectively. The nebulizing gas flow rate was 2 L / min.

example 3

[0202] Example 3 - Analysis of Tacrolimus in Whole Blood Samples

[0203] In this example, the PROSAD system was used to assay an immunosuppressant called tacrolimus (anti-rejection drug) contained in human plasma.

[0204] A binary chromatographic gradient was used comprising phase A) H2O + 0.05% formic acid and phase B) CH3CN + 0.05 formic acid. Given the time T=0 at the time of injection, %B was 15%. Maintain this condition for 2 minutes. After this interval, %B increased to a value of 70% within 8 minutes. Initial conditions were restored over the next two minutes. The instrument acquisition time was set at 24 minutes. A ThermolEctron C8 150×1 mm chromatographic column was used. The surface potential, electrospray potential and surface temperature were 50V, 0V and 110°C, respectively. The nebulizing gas flow rate was 2 L / min.

[0205] Finally, for the sake of completeness, for each of the above application examples, the % measurement accuracy error and the instrum...

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Abstract

Analysis system (10, PROSAD) for the quantitative chemical analysis of samples (A, A-1, A- 2,....), preferably but not solely in the medical field, comprising detection instrumentation or equipment (30) designed to detect the quantity of the target analytes ( [x], [y], [z] ) present in the various samples (A, A1) to be analysed, which in turn comprises a chromatography system (31), an ion source (32) and a mass spectrometer (33); a data processing system (40, 42, 43, 44) designed to process the quantitative data of the target analytes present in the samples analysed, as detected by the detection equipment (30); and an innovative database (41) containing corrective and control data and coefficients (K, C, K1) to be used to calibrate and correct the instrumental response of the specific detection equipment (30) used to analyse the samples, wherein said corrective and control data and coefficients are determined and acquired by the database at a preliminary stage (51, 52) before the actual analysis of the samples, wherein the sample to be analysed is prepared with a universal dilution solution in order to minimise the corresponding matrix effect, and wherein the data processing system determines the quantitative data of the target analytes present in the samples analysed by processing the quantitative data (Q) detected by the detection equipment, namely the mass spectrometer, taking account of the corrective and control data and coefficients contained in the database. By comparison with the analysis systems currently in use, the system for quantitative analysis of samples according to the invention presents the significant advantage of not requiring, in order to calibrate the detection equipment during the analysis of multiple samples, continuous and expensive use, for each analysis, of commercial standards of the target analytes to be quantitated.

Description

technical field [0001] The present invention relates generally to the field of quantitative chemical analysis systems and devices for samples, typically but not exclusively in the medical field, and more particularly to such analytical systems for quantitative chemical analysis of samples, characterized by a novel and advantageous calibration system, It is designed to calibrate the instrument response of a particular instrument or device in an analytical system that detects the amount of target analyte present in the various samples being analyzed. [0002] The invention also relates to a corresponding method for the quantitative analysis of samples, especially in the medical field, with calibration of the instrumental response of a particular instrument or device used to detect quantitative data of target analytes in the various analyzed samples. Background technique [0003] Modern analytical chemistry, in its practical application, needs to continuously enhance the quanti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J49/00
CPCH01J49/0036H01J49/0009G16H10/40
Inventor 马泰奥·弗洛里迪亚西莫内·克里斯托尼
Owner 马泰奥·弗洛里迪亚
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