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Method for preparing compound bacterium agent

A technology of composite bacterial agent and bacterial powder, applied in the direction of microorganism-based methods, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the constraints of large-scale probiotic products, single and stable bacterial action Improve feed remuneration rate, improve digestion and absorption rate, and easy operation

Inactive Publication Date: 2014-02-05
QINGDAO VLAND BIOTECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual industrial production, the number of viable bacteria is low, the function of the bacteria is single, and the stability is poor and strongly restricts the scale and industrialization of probiotic products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A composite microbial agent, which consists of bacillus subtilis, bacillus natto, bacillus coagulans, lactobacillus rhamnosus, clostridium butyricum, a carrier and a protective agent. The number of live bacteria of Bacillus subtilis was 10 8 cfu / g; the number of live bacteria of Bacillus natto is 10 9 cfu / g; the viable count of Bacillus coagulans is 10 8 cfu / g; the viable count of Lactobacillus rhamnosus is 10 8 cfu / g; the viable count of Clostridium butyricum is 10 5 cfu / g.

[0025] The carrier is shell powder and cornstarch with a mass ratio of 1:2.

[0026] The protective agent is trehalose and sodium glutamate with a mass ratio of 1:1.5.

[0027] In the above composite microbial agent, the mass ratio of the bacterial powder to the carrier and the protective agent is 4:1:0.5.

[0028] The method for the above-mentioned compound bacterial agent specifically comprises the steps:

[0029] 1) Single-strain fermentation: five strains of Bacillus subtilis, Bacillus ...

Embodiment 2

[0034] A composite microbial agent, which consists of bacillus subtilis, bacillus natto, bacillus coagulans, lactobacillus rhamnosus, clostridium butyricum, a carrier and a protective agent. The number of live bacteria of Bacillus subtilis was 10 9 cfu / g; the number of live bacteria of Bacillus natto is 10 10 cfu / g; the viable count of Bacillus coagulans is 10 7 cfu / g; the viable count of Lactobacillus rhamnosus is 10 7 cfu / g; the viable count of Clostridium butyricum is 10 6 cfu / g.

[0035] The carrier is shell powder and cornstarch with a mass ratio of 1:3.

[0036] The protective agent is trehalose and sodium glutamate with a mass ratio of 1:2.

[0037] In the above composite microbial agent, the mass ratio of the bacterial powder to the carrier and the protective agent is 4:1.5:1.

[0038] The method for the above-mentioned composite bacterial powder preparation specifically comprises the following steps:

[0039] 1) Single-strain fermentation: five strains of Bacil...

Embodiment 3

[0044]A composite microbial agent, which consists of bacillus subtilis, bacillus natto, bacillus coagulans, lactobacillus rhamnosus, clostridium butyricum, a carrier and a protective agent. The number of live bacteria of Bacillus subtilis was 3×10 8 cfu / g; the number of live bacteria of Bacillus natto is 6×10 9 cfu / g; the viable count of Bacillus coagulans is 5×10 7 cfu / g; the viable count of Lactobacillus rhamnosus is 8×10 7 cfu / g; the viable count of Clostridium butyricum is 3×10 5 cfu / g.

[0045] The carrier is shell powder and cornstarch with a mass ratio of 1:2.4.

[0046] The protective agent is trehalose and sodium glutamate with a mass ratio of 1:1.8.

[0047] In the above composite microbial agent, the mass ratio of the bacterial powder to the carrier and the protective agent is 4:1.2:0.6.

[0048] The method for the above-mentioned composite bacterial powder preparation specifically comprises the following steps:

[0049] 1) Single-strain fermentation: five st...

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Abstract

The invention aims to provide a method for preparing a compound bacterium agent for livestock breeding. The method comprises the following steps: fermenting bacillus subtilis, bacillus natto, bacillus coagulans, lactobacillus rhamnosus and clostridium butyricum, adding a carrier, performing plate-frame pressure filtration, adding a protective agent for emulsifying and spray drying, and finally, matching according to effective bacteria numbers of the five strains. The method is simple in process and easy to operate, the carrier and protective agent adopted in the preparation process are edible components, the safety of the bacterium agent can be guaranteed, and a reasonable proportion among the five strains can be guaranteed in the method, so that the strongest synergistic effect is achieved, and the method is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a composite bacterial agent for livestock and poultry breeding and a preparation method thereof. Background technique [0002] my country has now become a major country in the production and trade of livestock and poultry in the world. The production scale of poultry eggs ranks first in the world, and the output of poultry products ranks second in the world. The use of antibiotics causes a large amount of drug residues, resulting in the decline of poultry and livestock meat varieties, and the enhancement of bacterial drug resistance, which directly or indirectly affects human health. [0003] In recent years, probiotic products have become a hot spot as a green feed additive. Probiotics form dominant beneficial bacteria through biological competitive exclusion in the digestive tract of animals, and prevent and control the occurrence of diseases by enhancing animal immunity...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10A23K1/18A23K1/16C12R1/125C12R1/07C12R1/225C12R1/145
Inventor 李佩佩徐雪梅刘鲁民
Owner QINGDAO VLAND BIOTECH GRP
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