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Method for breaking wall of chlorella by using compound enzyme

A chlorella and compound enzyme technology, applied in the direction of microbial dissolution, etc., can solve the problems of complex composition, low wall-breaking rate, long time, etc., and achieve the effect of simple operation method, low equipment requirements, and mild operating conditions.

Active Publication Date: 2014-02-05
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because chlorella has a tough cell wall, even ruminants cannot digest its fiber and cannot eat it directly, and the extraction of its internal functional substances is also hindered, resulting in a certain degree of limitation in the widespread use of chlorella
The composition of the cell wall of Chlorella is complex, containing various polysaccharides such as cellulose and hemicellulose. The effect of hydrolyzing and breaking the wall with a single enzyme has not been ideal, and the highest rate of breaking the wall by enzymatic method can only reach 80%. Crude protein is easily destroyed
[0003] At present, the wall-breaking methods of chlorella mainly include repeated freezing and thawing, ultrasonic crushing, and ultrafine crushing. The repeated freezing-thawing method takes a long time and has a low wall-breaking rate; High, and it is easy to produce local high temperature phenomenon and cause oxidation of unsaturated fatty acids; ultrafine pulverization method requires professional equipment, and the processing capacity is low
Therefore, they are rarely used in large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1.1. Weigh 50 g of chlorella freeze-dried powder, add 2500 mL of distilled water to prepare chlorella dilution;

[0023] 1.2. Add 15.3 g of compound plant hydrolase with an enzyme activity of 10,000 U and 30.6 g of cellulase with an enzyme activity of 0.2 million U in the chlorella dilution;

[0024] 1.3. Add citric acid to adjust the pH value to 4;

[0025] 1.4. Heating at a constant temperature in a water bath at 30°C for 6 hours of enzymatic hydrolysis;

[0026] 1.5. Heat to 100°C to inactivate the enzyme, collect broken wall chlorella after cooling and fill it.

[0027] The detection of the broken wall rate of chlorella: dilute the broken chlorella 1000 times with distilled water, carry out microscope observation, according to the number of chlorella with complete form before and after broken wall, the broken wall rate of chlorella ( %)= ×100%, the calculated wall breaking rate of Chlorella was 88.6%.

Embodiment 2

[0029] 2.1, take by weighing chlorella freeze-dried powder 50g, add the citric acid solution 10000g that pH value is 6;

[0030] 2.2. Add 120.6g of compound plant hydrolase with an enzyme activity of 0.2 million U and 40.2 g of cellulase with an enzyme activity of 10,000 U in the chlorella dilution;

[0031] 2.3. Add citric acid to adjust the pH value to 6;

[0032] 2.4. Heating at a constant temperature in a 60°C incubator for enzymatic hydrolysis for 3 hours;

[0033] 2.5. Heat to 100°C to inactivate the enzyme, collect broken wall chlorella after cooling and fill it.

[0034] The detection of the broken wall rate of chlorella: dilute the broken chlorella 1000 times with distilled water, carry out microscope observation, according to the number of chlorella with complete form before and after broken wall, the broken wall rate of chlorella ( %)= ×100%, the calculated wall breaking rate of Chlorella was 84.5%.

Embodiment 3

[0036] 3.1. Weigh 50 g of chlorella freeze-dried powder, and add 7500 g of glacial acetic acid solution with a pH of 5;

[0037] 3.2. Add 60.4 g of compound plant hydrolase with an enzyme activity of 0.5 million U and 60.4 g of cellulase with an enzyme activity of 0.5 million U in the chlorella dilution;

[0038] 3.3. Add glacial acetic acid to adjust the pH value to 5;

[0039] 3.4. Heating at a constant temperature in a water bath at 45°C for 4.5 hours;

[0040] 3.5. Heat to 100°C to inactivate the enzyme, collect broken wall chlorella after cooling and fill it.

[0041] The detection of the broken wall rate of chlorella: dilute the broken chlorella 1000 times with distilled water, carry out microscope observation, according to the number of chlorella with complete form before and after broken wall, the broken wall rate of chlorella ( %)= ×100%, the calculated wall breaking rate of Chlorella was 90.5%.

[0042] Crude protein content wt% in chlorella before h...

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Abstract

The invention provides a method for breaking the wall of chlorella by using a compound enzyme. The method comprises the steps: diluting a chlorella raw material so as to obtain a chlorella diluent, adding composite plant hydrolase and cellulase, adjusting the pH value, heating at a constant temperature for carrying out enzymatic hydrolysis, heating for enzyme deactivation, cooling so as to collect wall-broken chlorella and filling. The method has the advantages that the operation method is simple, the operating conditions are mild, the pollution is avoided, the requirements on equipment are low, the handling capacity is large, the continuous operation can be carried out, the method is suitable for industrial production, the composite plant hydrolase and the cellulase are mainly used for the specificity degradation of the cell wall polysaccharide of chlorella, do not contain protease, can effectively retain the functional components of the chlorella such as the proteins and amino acids and have high wall-breaking rate, and the obtained wall-broken chlorella is green and safe and can be directly applied to the fields such as medicines, food, household necessities, environments and the like.

Description

technical field [0001] The invention relates to a method for breaking algae walls, in particular to a method for breaking walls of chlorella with compound enzymes. Background technique [0002] Chlorella is a universal single-celled green algae with high protein, mineral and vitamin content. Among them, the crude protein content can reach more than 50%, and it has good protein quality. At the same time, Chlorella is rich in Rich in unsaturated fatty acids, which mainly exist in the cytoplasm and cell membrane in the form of fatty acid glyceric acid and phospholipids, which play a significant role in preventing cardiovascular and cerebrovascular diseases, losing weight, and lowering blood lipids. Chlorella has been used as an additive and health food in foreign countries for more than 30 years. Currently, there are chlorella tablets and capsules, chlorella bread, and chlorella beverages that have been industrialized. In recent years, a lot of research work has been carried o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06
Inventor 朱丹实励建荣曹雪慧何余堂吴晓菲
Owner BOHAI UNIV
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