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Breeding, reproduction and seed production method for cytoplasmic male sterile line of Chinese flowering cabbage

A technology of male sterile lines and male sterility, applied in botany equipment and methods, horticultural methods, plant genetic improvement, etc., can solve problems such as low pollen amount, low-temperature yellowing nectaries, unstable sterility, etc. Achieve obvious advantages, high purity, and stable sterility

Active Publication Date: 2014-02-05
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The types of cytoplasmic male sterile lines of Chinese cabbage currently used in production are mainly rapeseed polima cytoplasmic male sterile (pol CMS) and radish cytoplasmic male sterile (ogu CMS), but rapeseed polima cytoplasmic male sterile (pol CMS) CMS) has the disadvantages of unstable sterility, low pollen quantity and low seed purity due to the influence of bad temperature; ogu CMS has the disadvantages of low-temperature yellowing and nectary degeneration, so excavating and creating a new type of cytoplasmic male sterility, It is of great significance to solve the single problem of cytoplasm and expand the existing germplasm resources

Method used

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  • Breeding, reproduction and seed production method for cytoplasmic male sterile line of Chinese flowering cabbage
  • Breeding, reproduction and seed production method for cytoplasmic male sterile line of Chinese flowering cabbage
  • Breeding, reproduction and seed production method for cytoplasmic male sterile line of Chinese flowering cabbage

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1 Breeding of Chinese cabbage cytoplasmic male sterile line

[0024] (1) Using hau CMS, a cytoplasmic male sterile material of Brassica juncea (hau CMS, which has been disclosed in the Chinese invention patent application number 200510086942.7, and the seed has been preserved in the China Type Culture Collection Center on November 12, 2005, and the preservation number is: CCTCC NO: P200504) was used as the female parent, and the maintainer line "Sijiu Caixin" (the seeds of this breeding material have been sold publicly before the application date) was used as the male parent for distant hybridization, cultivated by embryo rescue method, and transplanted in the field to obtain interspecific hybrids f 1 ;

[0025] (2) The interspecific hybrid F obtained by step (1) 1 Caixin BC1F was obtained by hybridizing the female parent with the maintainer Caixin "Sijiucaixin" and cultured from embryos 1 ;

[0026] (3) Using the choy sum BC1F obtained in step (2) 1 The fe...

Embodiment 2

[0039] Example 2 Propagation and seed production of cabbage cytoplasmic male sterile line 1301A

[0040] Under the isolation conditions of winter sowing in Wuhan, Hubei, China (from the end of November to early December every year) or summer sowing in Lanzhou, Gansu, China (in May every year), the cytoplasmic male sterile line 1301A of Caixin was used as the female parent, "Sijiu Caixin" As the male parent, the CMS line 1301A was propagated according to the row ratio (row number ratio) of 1:4 between the male parent and the female parent, and the other cultivation management was the same as that of ordinary Chinese cabbage.

[0041] The bred Chinese cabbage cytoplasmic male sterile line 1301A was crossed with "Zaoyou 1" to obtain the cabbage cytoplasmic male sterile hybrid variety.

[0042]Table 1 Comparison of phenotypes between Chinese cabbage cytoplasmic male sterile line 1301A and the new mustard hau CMS

[0043] Cytoplasmic male sterility type

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Abstract

The invention discloses a breeding, reproduction and seed production method for a cytoplasmic male sterile line of a Chinese flowering cabbage. The breeding, reproduction and seed production method comprises the following steps: (1) taking a leaf mustard type oilseed rape cytoplasmic male sterility material hau CMS as a female parent and taking a Chinese flowering cabbage conventional variety brassica parachinensis bariley as a male parent; carrying out distant hybridization; separating hybridized strains and carrying out duplicitas rescuing culture; transplanting to a large field to obtain a species hybrid F1; and (2) selecting a sterile strain in the species hybrid F1 as a female parent and taking the brassica parachinensis bariley as a recurrent parent to continuously backcross for 5 generations to obtain the cytoplasmic male sterile line 1301A of the Chinese flowering cabbage. The cytoplasmic male sterile line of the Chinese flowering cabbage obtained by breeding has the characteristics that the sterility is stable and complete and is not influenced by environment, and leaves are not yellowed and the like; continuous planting of 5 generations to 10 generations is carried out and fertility identification is carried out; the sterility degree and the sterile strain rate of the sterile line are 100%.

Description

technical field [0001] The invention belongs to the technical field of vegetable hybrid breeding, and in particular relates to a method for breeding, breeding and seed production of a cytoplasmic male sterile line of Chinese cabbage. Background technique [0002] There is obvious heterosis in cruciferous vegetables, and the main ways to utilize heterosis are self-incompatibility and male sterility, among which cytoplasmic male sterility is an ideal pollination control system. By using male sterile types from different sources, we selected and bred Chinese cabbage cytoplasmic male sterile lines that are stable and thorough under different light and temperature conditions, and then bred and utilized them to prepare the first generation of male sterile hybrids with excellent comprehensive traits. . The first-generation utilization of cruciferous vegetable hybrids has been widely carried out, especially in Asian countries, such as China, Japan, and South Korea. At present, self...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
Inventor 万正杰张甜徐跃进李金萍刘利艳袁贞贞马智宏许乃凡
Owner HUAZHONG AGRI UNIV
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