Electrochemical detection method for gene mutation of ribosyltransferase

Abstract

The invention discloses an electrochemical detection method for gene mutation of ribosyltransferase, which relates to an electrochemical detection method for gene mutation and aims to solve the problems of complicated measures, high cost and large errors which are caused by a fact that a result can only be determined by detecting a genetic endpoint by an existing method in detection of gene mutation of cell strains and the problem that the health of a tester can be injured. The electrochemical detection method for the gene mutation of ribosyltransferase comprises the steps of 1, preparing a nano composite working electrode; 2, respectively detecting electrochemical signals of a cell lysis solution, purine base monomers and a purine base mixed solution through the prepared nano composite working electrode, and analyzing and determining a peak on which the purine base electrochemical signal is located; and 3, performing electrochemical detection every 12 hours after cells are mutated through a mutagenesis agent, and constructing an HGPRT (hypoxanthine-guanine phosphoribosyl transferase) gene mutation electrochemical test method by taking electrochemical signal changes at different moments as indexes. The technical scheme provided by the invention is used in the field of electrochemical detection methods.

Description

Technical field [0001] The invention relates to an electrochemical detection method of gene mutations in mammalian cell lines, in particular to electrochemical detection of gene mutations in ribose transferase gene sites. Background technique [0002] Mammalian cell mutagenicity test is an important traditional international method for evaluating mutagenic factors. It uses mammalian cells as materials to determine the mutagenicity of the test substance by detecting mutations in specific genes in the cells. Hypoxanthine guanine phosphoribosyl transferase (HGPRT) gene is an ideal mutation biomarker. As an endogenous structural gene, it encodes a key enzyme for purine metabolism salvage pathway, because it is sensitive to various mutagenic factors The advantages of low spontaneous mutation rate and so on have become important target genes in gene mutation research. In the HGPRT gene mutation test in mammalian cells, HGPRT gene mutation will cause the key enzyme in the purine metabo...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing method can only determine the gene mutation result by detecting the genetic end point when detecting the gene mutation of the traditional mammalian cell line. False-negative results caused by incompleteness, and fluorescent and radioactive labels are required in the test, which will cause harm to the health of test personnel, and provide an electrochemical detection method for ribosyltransferase gene mutation

Images