Method for producing silkworm chrysalis peptide powder and silkworm chrysalis meal by aqueous biological enzyme method

Abstract

The invention relates to a method for producing silkworm chrysalis peptide powder and silkworm chrysalis meal by an aqueous biological enzyme method. According to the method, silkworm chrysalises serve as raw materials, an enzymolysis solution is obtained through drying, crushing and carrying out aqueous-phase enzymolysis, a supernatant and residues are obtained through carrying out centrifugation on the enzymolysis solution, the silkworm chrysalis meal is obtained through drying and crushing the residues, and the silkworm chrysalis peptide powder is obtained through carrying out oil-water separation, ultrafiltration-membrane ultrafiltration and desalination on the supernatant and then drying and crushing. The method specifically comprises the following steps of (1) drying and crushing; (2) carrying out aqueous-phase enzymolysis; (3) carrying out centrifugation and oil-water separation; (4) carrying out ultrafiltration-membrane filtration; (5) carrying out desalination; (6) drying and crushing, thereby obtaining the silkworm chrysalis peptide powder. According to the method, the aqueous biological enzyme method is applied to insect cells-silkworm chrysalis cells for the first time; the method has the advantages that the process is simple, safe and environmental-friendly, the cost is low, the quality of the obtained silkworm chrysalis peptide powder and silkworm chrysalis meal is high, and the like; the method disclosed by the invention has the characteristics that the oil and fat removal efficiency is high, the residual fat content of the obtained silkworm chrysalis peptide powder and silkworm chrysalis meal is low, the oligopeptide content of the obtained silkworm chrysalis peptide powder and silkworm chrysalis meal is high, and the like.

Description

technical field [0001] The invention relates to a method for producing silkworm chrysalis peptide powder and silkworm chrysalis meal by an aqueous biological enzyme method, belonging to the technical field of bioengineering. Background technique [0002] my country is a big country with a long history of silkworm rearing, silk reeling and silk making. The annual production of dried silkworm chrysalis in industrial production is about 200,000 tons. With such a huge amount of silkworm chrysalis resources, except for a small part of them are processed for food, most of them are directly baked. Dried and crushed for feed. The oil content of dried silkworm chrysalis is about 28.9~33.1%. The storage period of silkworm chrysalis is very short, and it is easy to cause rancidity or even mildew when stored for a long time. At present, silkworm chrysalis resources after reeling are directly used as feed. On the one hand, a large amount of oil resources are wasted, and it is easy to cau...

AI Technical Summary

Problems solved by technology

However, there are mainly two problems as follows: on the one hand, the price of silkworm chrysalis defatted powder is very high, with an average of 18,000 yuan / ton, and the production cost is high; There will be residues of organic solvents involved, which will also bring huge pressure on production, environmental protection and safety.

[0004] Aqueous bio-enzyme method is an emerging ideal method for hydrolyzing oleaginous protein. It breaks the plant cell wall through enzymatic hydrolysis, releases the oil, retains the biological activity of the oil, and has mild action conditions, simple process route, and energy consumption in the production process. Low, but there are difficulties in research and development, low oil yield, and difficulty in demulsification of the emulsion formed by protein and oil after enzymatic hydrolysis. The research on water-phase biological enzyme method is currently a hot spot in the food industry at home and abroad. So far A variety of oils have been tested, but most of them are limited to plant cells, and no relevant research reports have been found on insect cells with high fat content

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