A kind of method for producing gla by fermenting and producing gla by cultivating Xiao Ke Yinhan at a high density

A high-density culture and Yinhan technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long production cycle and unstable oil production, and achieve low production costs and acceptable product quality. Good controllability and the effect of reducing the occupation of cultivated land

Active Publication Date: 2016-03-09
XIAMEN HUISON BIOTECH
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Problems solved by technology

Plants rich in γ-linolenic acid are mostly wild. Due to the influence of climate, production area and other conditions, there are problems such as unstable oil production and long production cycle. Therefore, extracting GLA from plants cannot meet the market demand.

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0024] The first step, according to glucose: 50g / L, KH 2 PO 4 : 2g / L, MgSO 4 ·7H 2 00.5g / L configuration activation medium, 121 ℃, 25min sterilization standby;

[0025] Inoculate 2mL of Xiaoke Yinhan glycerol tubes stored in ultra-low temperature refrigerators into 250mL first-level shake flasks containing 50mL of activation medium, and activate and culture them in a shaker at 22°C at 170rpm for 48h;

[0026] In the second step, according to the inoculum amount of 10% volume percentage, inoculate the activated cultured Xiaoke Yinhan bacteria into the secondary shaker flask containing 200mL of sterilized activation medium, and expand the culture in a shaker at 22°C with a rotation speed of 170rpm 48h;

[0027] The third step, press glucose in 1000L seed tank: 100g / L, KH 2 PO 4 : 8g / L, MgSO 4 ·7H 2 O: 4g / L, yeast extract: 20g / L; NaNO 3 : 5g / L to prepare 500L seed tank culture medium, after the preparation is completed, sterilize at 121°C for 30 minutes for later use;

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Abstract

The invention discloses a method for producing gamma linolenic acid (GLA) through fermenting high-density cultured cunninghamellaceae. The method comprises the following steps: according to a volume ratio of preserved glycerin strain to shake flask activation culture medium being 1:25, inoculating a cunninghamellaceae strain to a first-level shake flask which contains an activation culture medium and carrying out activation cultivation; according to an inoculum size of 5-10vol%, transferring the cunninghamellaceae strain to a second-level shake flask for enlarging cultivation; according to the inoculum size of 5-10vol%, inoculating the cunninghamellaceae strain subjected to cultivation enlargement into a first-level seeding tank for fermentation; and according to the inoculum size of 5-10vol%e, inoculating the cunninghamellaceae strain subjected to cultivation enlargement in the seeding tank into a fermenting tank for fermentation cultivation, and after finishing the fermentation, measuring that the dry weight of hyphae in a fermentation liquor reaches 60-80g / L, the oil content reaches 20-25g / L and the GLA content reaches 4.8-6.1g / L. The method can be used for lowering the production cost of GLA and is suitable for the industrial production of GLA.

Description

technical field [0001] The invention relates to a production method of GLA, in particular to a method for producing GLA by high-density cultivating Xiaoke Yinhan and fermenting GLA. The method adopts microbial fermentation to produce GLA, and can be applied to large-scale industrial production of GLA. Background technique [0002] γ-linolenic acid (GLA) is a polyunsaturated fatty acid belonging to the n-6 series. The relative molecular weight of GLA is 278.4. It is liquid at room temperature, insoluble in water but easily soluble in ethanol, ether, chloroform, petroleum In non-polar solvents such as ether, when the purity of GLA reaches more than 35%, it is very easy to oxidize and is not easy to store, and can only exist in the state of dry oil. [0003] GLA has many important physiological functions. GLA is one of the essential fatty acids in the human body. It is one of the important components of the biofilm of various tissues in the human body. It undergoes complex meta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 陈礼毅钟惠昌
Owner XIAMEN HUISON BIOTECH
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