Method for detecting relative gene polymorphism of warfarin personalized medication by using high-resolution melting curve analysis technology

Abstract

The invention discloses a method for detecting relative gene polymorphism of warfarin personalized medication by using a high-resolution melting curve analysis technology. The method is characterized by comprising the following steps: adopting a silica gel adsorption method to extract genome DNA (Deoxyribose Nucleic Acid) of an oral epithelial cell / peripheral blood cell sample of a detected person; designing a detection primer containing a target site; carrying out site typing on a PCR (Polymerase Chain Reaction) amplified gene sequence by using the high-resolution melting curve (HRM) analysis technology. The method disclosed by the invention is high in sensitivity, good in specificity and fast in detection speed, and can be used for providing reference to a clinical medication dosage of warfarin, so as to realize reasonable and effective personalized medication.

Description

technical field [0001] The invention relates to a method for detecting gene polymorphisms related to individualized medicine of warfarin, and belongs to the technical field of molecular biology. Background technique [0002] Warfarin is a commonly used anticoagulant drug in clinical practice. It inhibits the formation of new thrombus by inhibiting the activation of coagulation factors, restricts the expansion and extension of thrombus, inhibits the occurrence of thrombus shedding and embolism, and is beneficial to the removal of thrombus. Clinically, it is mainly used for the treatment of atrial fibrillation, heart valve repair, recurrent stroke, deep vein thrombosis and pulmonary embolism. The pharmacology of warfarin is complex, and even small dose-response changes can lead to thrombosis or bleeding. The clinical dosage can vary by about 20 times. How to use warfarin correctly and monitor and adjust the dosage reasonably has become a difficult problem for clinicians. War...

AI Technical Summary

Problems solved by technology

This method has few steps, is not easy to pollute, and is convenient for typing large samples; however, it has certain restrictions on primer design and certain requirements on the sequence near the SNP, which is limited by the position and type of the mutated base

Direct DNA sequencing can directly provide accurate base information and is regarded as the gold standard for mutation detection, but it is time-consuming, laborious, expensive, and not suitable for detection of a large number of samples.

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