Method for producing lycopene through fermentation

Abstract

The invention provides a method for producing lycopene by fermentation. The method adopts B. trispora (+) and B. trispora (-) strains and cultures on an inclined plane: in an incubator at 26-28°C Medium culture for 6-7 days; after seed culture for 36-46h, ferment culture, add betaine for the first time, ferment at 26-28°C, 200-250r / min for 100-120h, add blocking at 42h agent, while adding betaine for the second time until the end of fermentation. The wet thallus is collected by filtering the fermented liquid, and the dry thallus containing lycopene is obtained after vacuum freeze-drying. Adding betaine in the present invention can effectively promote the respiratory chain system of the bacteria and increase the oxygen consumption rate of the bacteria; at the same time, it can effectively alleviate the inhibition of osmotic pressure respiration of the bacteria in the fermentation process, accelerate the oxygen transfer rate, and reduce the energy consumption of the bacteria. consumption; the growth rate of bacteria and the content of lycopene are improved, and the production cost is reduced. The method of the invention is simple to operate, the betaine has no toxic and side effects, is convenient and safe to use, is suitable for industrial production, and has great application value.

Description

technical field [0001] The invention relates to biological engineering, in particular to fermentation engineering, in particular to a method for producing lycopene by fermentation. Background technique [0002] Lycopene is a carotenoid, a natural pigment contained in plants, mainly found in the ripe fruit of tomato, a plant in the family Solanaceae. Studies have proved that lycopene has the effects of improving human immunity, anti-cancer, anti-oxidation, lowering blood fat, and protecting the skin. As people pay more and more attention to greenness and health, lycopene has been widely concerned by people. However, as a functional natural pigment, lycopene cannot be synthesized by human beings and needs to be supplemented through diet and other methods. According to the forecast of CMR (Customer Management Relationship) International Company in the United States, the sales of lycopene products will increase by 35% annually, and the sales volume is expected to reach 20 billi...

AI Technical Summary

Problems solved by technology

The disadvantage of the current production of lycopene by microbial fermentation is that microorganisms cannot accumulate lycopene at a high level, resulting in low fermentation yield and high production costs

B. trispora is a highly aerobic bacterium, and the fermentation process has a great demand for oxygen; the medium used has high solid content and high viscosity, which increases the osmotic pressure of the external environment where the bacteria are located, and the medium It also contains about 8% vegetable oil (providing double bonds for metabolism), resulting in a low dissolved oxygen rate in the fermentation system; at the same time, the production process is often affected by factors such as the speed of rotation. Abnormal, decreased biomass and metabolites

However, if the concentration of liquid alkanes is too high, it will cause damage to the bacteria, and the later separation and extraction must also undergo detoxification treatment, which is cumbersome to operate

The addition of activated carbon also has problems such as increased power loss of equipment, easy blockage of pipelines, cumbersome pre-treatment and post-advanced procedures, etc.