Method for producing lycopene through fermentation

Abstract

The invention provides a method for producing lycopene through fermentation. According to the invention, blakeslea trispora (+) and blakeslea trispora (-) strains are adopted, and slant culture is carried out. Culturing is carried out for 6-7 days under a temperature of 26-28 EG C in an incubator; after 36-46h of seed culturing, fermentation culture is carried out; betaine is added for a first time, and fermentation culture is carried out for 100-120h under a temperature of 26-28 DEG C and under a rotation speed of 200-250r / min; a blocking agent is added when fermentation is carried out for 42h, and betaine is added for a second time; and fermentation is continued until finished. A fermentation broth is filtered, and wet thalli are collected. Through vacuum lyophilization, dried thalli comprising lycopene are obtained. According to the invention, with the added betaine, thalli respiratory chain system can be effectively promoted, and thalli oxygen consumption rate can be improved. Also, osmotic pressure respiratory depression upon the thalli during the fermentation process can be effectively relieved, oxygen delivery rate can be accelerated, and energy consumption can be reduced. thalli growth rate and lycopene content are improved, and production cost is reduced. According to the method, the operation is simple. Betaine has no toxic or side effect, and is convenient and safe to use. The method is suitable for industrialized productions, and has high application values.

Description

technical field [0001] The invention relates to biological engineering, in particular to fermentation engineering, in particular to a method for producing lycopene by fermentation. Background technique [0002] Lycopene is a carotenoid, a natural pigment contained in plants, mainly found in the ripe fruit of tomato, a plant in the family Solanaceae. Studies have proved that lycopene has the effects of improving human immunity, anti-cancer, anti-oxidation, lowering blood fat, and protecting the skin. As people pay more and more attention to greenness and health, lycopene has been widely concerned by people. However, as a functional natural pigment, lycopene cannot be synthesized by human beings and needs to be supplemented through diet and other means. According to the forecast of CMR (Customer Management Relationship) International Company in the United States, the sales of lycopene products will increase by 35% annually, and the sales volume is expected to reach 20 billion...

AI Technical Summary

Problems solved by technology

The disadvantage of the current production of lycopene by microbial fermentation is that microorganisms cannot accumulate lycopene at a high level, resulting in low fermentation yield and high production costs

B. trispora is a highly aerobic bacterium, and the fermentation process has a great demand for oxygen; the medium used has high solid content and high viscosity, which increases the osmotic pressure of the external environment where the bacteria are located, and the medium It also contains about 8% vegetable oil (providing double bonds for metabolism), resulting in a low dissolved oxygen rate in the fermentation system; at the same time, the production process is often affected by factors such as the speed of rotation. Abnormal, decreased biomass and metabolites

However, if the concentration of liquid alkanes is too high, it will cause damage to the bacteria, and the later separation and extraction must also undergo detoxification treatment, which is cumbersome to operate

The addition of activated carbon also has problems such as increased power loss of equipment, easy blockage of pipelines, cumbersome pre-treatment and post-advanced procedures, etc.