A kind of malic enzyme gene sgme1 and its application

A malic enzyme gene and malic enzyme technology, applied in the field of plant biology, can solve problems such as aluminum stress

Inactive Publication Date: 2016-01-20
SOUTH CHINA AGRI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Through the early screening of aluminum tolerance, we found that the phosphorus-inefficient Stylo genotype Fine-stem is sensitive to aluminum stress, and the phosphorus-efficient Stylo genotype TPRC2001-1 has good tolerance to aluminum toxicity (Due et al., 2009), and The aluminum tolerance of TPRC2001-1 is equivalent to that of rice, but there are few studies on the mechanism of aluminum tolerance of stylophyllum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of malic enzyme gene sgme1 and its application
  • A kind of malic enzyme gene sgme1 and its application
  • A kind of malic enzyme gene sgme1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] SgME1 Gene cloning

[0052] 1. Isolation and identification of SgME1 protein

[0053] Refer to Chen Zhijian et al. (2011) method to extract Stylosanthes root protein and perform two-dimensional electrophoresis. Three replicates were set for each experimental treatment, and the protein spots that were significantly differently expressed between the non-aluminum control and the aluminum treatment were screened, and MALDI-TOF / TOFMS mass spectrometry analysis was performed with ABI4700TOF-TOF (Applied Biosystems, USA).

[0054] The result is Figure 4 As shown, it can be seen from the results that, compared with the control, the accumulated amount of SgME1 protein in the roots of Stylosanthes after 24 hours of aluminum stress did not change significantly. However, after 72 hours of aluminum stress, the accumulation of SgME1 protein increased significantly in the roots, indicating that the expression of SgME1 protein was enhanced by long-term aluminum stress.

[0055] 2. SgME1 Gene...

Embodiment 2

[0087] Research on genetically modified materials

[0088] 1. Obtaining genetically modified materials

[0089] (1) Obtaining yeast transgenic strains

[0090] With reference to SCeasycomptransformationkit (Invitrogen, USA), pYES2- SgME1 The empty vector of pYES2 was transformed into yeast INVSC1.

[0091] (2) Obtaining of genetically modified kidney bean hairy roots

[0092] The constructed expression vector was transformed into Agrobacterium rhizogenes K599, and the transgenic hairy roots were obtained by Agrobacterium-mediated induction of bean hairy roots in vitro.

[0093] (3) Obtaining transgenic Arabidopsis plants

[0094] The constructed expression vector plasmid was transformed into Agrobacterium tumefaciens Gv3101, and Agrobacterium-mediated Arabidopsis inflorescence transfection was used to obtain transgenic Arabidopsis plants.

[0095] 2. Detection of genetically modified materials

[0096] (1) Detection of yeast transgenic strains

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a gene encoding malic enzyme SgME1 and its application. the malic enzyme gene SgME1 The nucleotide sequence such as SEQ? ID? As shown in NO:1, the amino acid sequence of the encoded protein is shown as SEQ? ID? NO:2 shown. SgME1 Genes regulating stylomalate synthesis, overexpressed SgME1 Promotes malic acid synthesis and increases aluminum tolerance in yeast, bean hairy root and Arabidopsis thaliana.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to malic enzyme gene SgME1 and its application. Background technique [0002] Aluminum toxicity is one of the main obstacles to crop growth on acidic soils (Kochian et al., 2004). When the pH is less than 5, the aluminum in the soil exists in an active form, which can be quickly absorbed by the plant roots (Kinraide, 1991), and has a toxic effect on the growth of the root tips, eventually leading to a decrease in crop yield. [0003] Aluminum ions can interact with cell walls and symplasts, thereby disturbing the normal physiological and biochemical reactions of plant cells (Kochian et al., 2005). Studies have shown that aluminum is easily bound to the negatively charged cell wall pectin, thereby reducing the extensibility of the cell wall and limiting cell elongation (Yang et al., 2011). In addition to combining with the cell wall, aluminum can also accumulate in the symplast,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/04C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/82A01H5/06A01H5/00
Inventor 田江廖红孙丽莉
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap