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Application of alanine as vaccine adjuvant

A vaccine adjuvant and alanine technology, applied in the field of biomedicine, can solve problems such as adjuvant disease, and achieve the effect of not causing toxic and side effects

Inactive Publication Date: 2015-11-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The most classic vaccine adjuvant at present is Freund's adjuvant, but this adjuvant is mainly used in animal experiments, but adjuvant disease often occurs after repeated injections in animals

Method used

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  • Application of alanine as vaccine adjuvant

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Effect test

Embodiment 1

[0018] The cultivation and processing of embodiment 1 Edwardsiella tarda EIB202 bacterial strain

[0019] Pick the single clone of Edwardsiella tarda EIB202 on the Tryptone Soy Broth (TSB) plate in a TSB test tube, culture at 30°C for 24 hours, transfer to 100mL TSB medium at a ratio of 1:100 to expand the culture. When the OD600 value reached 1.0, the bacteria were collected by centrifugation, washed twice with normal saline, and the bacteria were suspended with normal saline to make the OD600 1.0 (the number of bacteria was about 109 bacteria / ml), which was used to challenge the experimental mice.

[0020] At the same time, take another part of the bacteria, add 0.1% formaldehyde, and incubate at 30°C for 24 hours to inactivate the bacteria. Then wash with normal saline for 3 times, suspend with normal saline to make OD600 1.0 (about 109cfu / mL), and then dilute 10 times with normal saline (about 108cfu / mL) to get the inactivated bacterial vaccine, which is used for experimen...

Embodiment 2

[0021] The immunization of embodiment 2 mice

[0022] Ninety 4-week-old Kunming mice (average body weight 25g) were randomly divided into 3 groups: control group, inactivated bacteria immunization group and inactivated bacteria + alanine immunization group. Each mouse in the control group was intraperitoneally injected with 100 μL of normal saline; each mouse in the inactivated bacteria immunization group was injected intraperitoneally with 100 μL of the prepared inactivated bacteria vaccine (about 10 7 bacteria); in the inactivated bacteria + alanine immunization group, each mouse was intraperitoneally injected with the mixture of 100 μL of the prepared inactivated bacteria vaccine and 120 μmoL of alanine. Two consecutive immunizations with an interval of 7 days. Seven days after the last immunization, the virus was challenged with EIB202 at a dose of 3×10 8 CFU / only, observed for 15 days, and counted the death situation of the mice. Calculate the relative immune protectio...

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Abstract

The invention relates to the field of biological medicines, and particularly discloses application of alanine serving as a vaccine adjuvant. According to the application of alanine, the alanine is added or not added into a vaccine to discover that the death rate an experimental animal is significantly reduced after the alanine is added in comparison with that without the alanine, which shows that the alanine can be used for improving the immune effect of the vaccine and can be used as an immunologic adjuvant of the vaccine.

Description

technical field [0001] The invention relates to the field of biomedicine, more specifically, relates to the application of alanine as a vaccine adjuvant. Background technique [0002] Alanine is the basic unit of protein and one of the 21 amino acids that make up human protein. Its molecular formula is C 3 h 7 o 2 N, it can prevent kidney stones, assist glucose metabolism, help alleviate hypoglycemia, and improve body energy. [0003] Vaccine adjuvants are substances that are injected into the body together with the antigen or in advance, which can enhance the body's immune response to the antigen or change the type of immune response. The most classic vaccine adjuvant at present is Freund's adjuvant, but this adjuvant is mainly used in animal experiments, but adjuvant disease often occurs after repeated injections in animals. Therefore, it is of great application value to find new immune adjuvants that are safe, effective and will not cause toxic or side effects. Con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61P37/04
Inventor 彭宣宪李惠王超
Owner SUN YAT SEN UNIV
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