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Application of anti-CiP antibody in diagnosis of early rheumatoid arthritis

A technology of glycine and arginine, which can be used in material inspection products, biological tests, peptides, etc., and can solve problems such as unclear etiology

Active Publication Date: 2014-01-15
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the etiology of RA is still unclear, and the antigens of pathogens may trigger the autoimmune response of RA through molecular mimicry mechanism due to the structure similar to the body's own proteins

Method used

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  • Application of anti-CiP antibody in diagnosis of early rheumatoid arthritis
  • Application of anti-CiP antibody in diagnosis of early rheumatoid arthritis
  • Application of anti-CiP antibody in diagnosis of early rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Design and synthesis of anti-CiP

[0014] Select the 345th-362th amino acid sequence of HPV47 type transcription regulator protein E2 protein (E2 345-362 ), replace the arginine at position 348 with citrulline, synthesize the CiP antigen polypeptide, synthesize it by solid-phase synthesis, and purify it by high-performance liquid chromatography.

[0015] Its sequence is as follows: KRGNT X GRGRGRQGRAGSSG

[0016] (i.e. Lys-Arg-Gly-Asn-Thr-Cit-Gly-Ary-Gly-Arg-Gly-Arg-Gln-Gly-Arg-Ala-Gly-Ser-Ser-Gly Lysine-Arginine-Glycine Amino Acid-Asparagine-Threonine-Citrulline-Glycine-Arginine-Glycine-Arginine-Glycine-Arginine-Glutamine-Glycine-Arginine-Alanine-Glycine-Serine -serine-glycine). Studies have shown that the above-mentioned anti-CiP antibody has a significantly higher antibody titer than other related diseases in the serum of early RA patients, and has better sensitivity and specificity, which is of great significance for the diagnosis of early RA.

Embodiment 2

[0017] Example 2 Detection of anti-CiP antibodies in the serum of early RA patients

[0018] In this study, enzyme-linked immunosorbent assay (ie, ELISA) was used to detect early RA, systemic lupus erythematosus, Sjogren's syndrome, osteoarthritis, dermatomyositis / polymyositis, systemic sclerosis and normal human serum anti-inflammatory CiP antibodies were detected to evaluate the distribution of the peptide antibodies in various diseases.

[0019] First, CiP is coated on an ELISA microwell plate and incubated with the serum to be tested. If there is an anti-CiP antibody in the serum, the antibody will bind to the coated solid-phase antigen to form an antigen-antibody complex. . After washing the unbound antibody and other components, add horseradish peroxidase (HRP)-labeled goat anti-human IgG antibody (IgG-HRP) and incubate together. At this time, IgG-HRP will bind to the antigen-antibody complex Combine to form antigen-antibody-enzyme-labeled antibody complex, wash off un...

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Abstract

The name of the invention is application of an anti-CiP antibody in diagnosis of an early rheumatoid arthritis. The invention provides a method for CiP and analogues thereof for detection of the early rheumatoid arthritis (RA). A polypeptide antigen can be specifically combined with a specific antibody inside a body of a sufferer with the early RA, the anti-CiP antibody has high sensitivity and specificity, and has high positive rate in the sufferer with the early RA having negative IgM-RF or anti-CCP antibody, and detection results of an anti-keratin antibody (AKA) and an antiperinuclear factor (APF). Auxiliary diagnosis of the sufferer with the negative IgM-RF antibody or the negative anti-CCP antibody is facilitated.

Description

technical field [0001] The invention relates to the application of anti-CiP antibody in detecting early rheumatoid arthritis. 1. Background technology [0002] Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by multi-joint erosive synovial inflammation, with a prevalence rate of 0.33%. There are about 5 million patients in my country, and most of them are young and middle-aged women , without formal treatment, the disability rate reaches 75%. In the second national sampling survey of disabled people in my country in 2006, disabling joint disease ranked second (20.1%) among the 21 causes of physical disability, second only to cerebrovascular disease (20.6%). 79.4% of them were disabled by RA. RA progresses rapidly, and bone destruction is the fastest in the first year, so accurate diagnosis of early RA is very important. [0003] A large number of studies in recent years have shown that citrullinated modification is of great significance in the patho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08G01N33/68
Inventor 栗占国孙晓麟李桂叶
Owner PEOPLES HOSPITAL PEKING UNIV
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