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Punica granatum peel antitumor polyphenol effective part, its preparation method and application

A technology of effective parts and pomegranate peel, applied in the field of medicine and pharmacy, can solve problems such as waste of resources

Inactive Publication Date: 2014-01-15
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the literature research shows that: the current application technology of pomegranate mainly focuses on the extraction and utilization of pomegranate juice, and the pomegranate peel, which still contains a large amount of polyphenols after the juice is squeezed, is discarded as food processing waste, causing serious problems. waste of resources

Method used

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  • Punica granatum peel antitumor polyphenol effective part, its preparation method and application
  • Punica granatum peel antitumor polyphenol effective part, its preparation method and application
  • Punica granatum peel antitumor polyphenol effective part, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: through the following steps:

[0035] a) After pulverizing 30 g of pomegranate peel, add 240 ml of 80% ethanol as an extraction solvent according to the weight-to-volume ratio of material to liquid: 1:8, extract at 5° C. for 10 min, and filter.

[0036] b) Concentrate the extract prepared in step a) under reduced pressure at 0.4 MPa and 70° C. to a suspension containing 30 mg of crude drug per 1 mL.

[0037] c) Add the concentrated solution prepared in step b) to HPD500 macroporous adsorption resin, adjust the pH value of the sample solution to 2 through 1.2mol / L hydrochloric acid, and the sample volume is 2BV, and the sample is loaded at an adsorption rate of 2BV / h, and the adsorption time is For 3 hours, the resin was eluted with 8BV of distilled water, followed by 6BV of 30% ethanol and 6BV of 50% ethanol at an elution rate of 2BV / h. The ethanol eluate was collected and concentrated under reduced pressure at 0.4MPa and 70°C.

[0038] d) Put the concent...

Embodiment 2

[0041] Embodiment 2: The difference from Implementation 1 is: in step a), after pulverizing 30 g of pomegranate peel, add 3000 ml of 80% ethanol as an extraction solvent at a weight-to-liquid ratio of 1:100, extract at 70° C. for 100 min, and filter.

[0042] The obtained effective fraction was 8.1 g, and the yield was 27%. Through content determination, the sum of the weights of polyphenolic components accounts for 50% of the total weight of the effective part, and the weights of ellagic acid, gallic acid and punicalagin in the polyphenolic components account for 10% and 0.80% of the total weight of the effective part, respectively. %, 35%. Check by the chromatographic conditions of Example 1, the chromatographic behavior meets image 3 The HPLC fingerprint of the effective fraction of polyphenols in pomegranate peel is shown.

Embodiment 3

[0043] Embodiment 3: through the following steps:

[0044] a) Grind 30 g of pomegranate peel and pass through a 40-mesh sieve, add 300 ml of 30% ethanol as an extraction solvent according to the weight-to-volume ratio of material to liquid 1:10, extract at 10° C. for 15 min, and filter.

[0045] b) Concentrate the extract prepared in step a) under reduced pressure at 0.4 MPa and 50° C. to a suspension containing 10 mg of crude drug per 1 mL.

[0046] c) Add the concentrated solution prepared in step b) to HPD500 macroporous adsorption resin, adjust the pH value of the sample solution to 2 through 1.2mol / L hydrochloric acid, and the sample volume is 2BV, and the sample is loaded at an adsorption rate of 1BV / h, and the adsorption time is For 3 hours, the resin was eluted with 6BV of distilled water, followed by 6BV of 30% ethanol and 3BV of 50% ethanol at an elution rate of 1BV / h. The ethanol eluate was collected and concentrated under reduced pressure at 0.4MPa and 50°C.

[00...

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Abstract

The invention discloses a punica granatum peel antitumor polyphenol effective part, which is obtained by subjecting a punica granatum peel ethanol extract to macroporous resin separation and purification. Specifically, the total weight of polyphenol components accounts for 50-80% of the total weight of the effective part, and the weights of ellagic acid, gallic acid and Punicalagin respectively account for 10-25%, 0.80-1.2% and 35-53% of the effective part. The punica granatum peel antitumor polyphenol effective part makes full use of the characteristic that punica granatum peel resources and the active component content meet the requirements for adjunctive treatment and / or prevention of tumors. The invention also discloses a preparation method of the punica granatum peel antitumor polyphenol effective part and application of the punica granatum peel antitumor polyphenol effective part in preparing pharmaceutical preparations for adjunctive treatment and / or prevention of tumors.

Description

technical field [0001] The invention relates to the technical field of medicine and pharmacy, in particular to a pomegranate skin anti-tumor polyphenol effective fraction. The invention also discloses the preparation method and application of the anti-tumor polyphenol effective part of the pomegranate peel. Background technique [0002] Tumor is the most common and frequently-occurring disease that threatens human health, and its pathogenesis has many factors. In recent years, the incidence of neoplastic cancer in my country has shown an obvious upward trend. At present, the main treatment methods include surgery, radiotherapy, chemotherapy, endocrine therapy and targeted therapy, each of which has advantages and limitations. Finding an effective, low-toxic, and widely applicable tumor-suppressing cancer drug has become a current research hotspot. [0003] Polyphenols have a wide range of biological effects in mammalian cells. A large number of epidemiological studies and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61K131/00
Inventor 高晓黎马桂芝常占英姜靖丁楠王春梅
Owner XINJIANG MEDICAL UNIV
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