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Anti-nematode soybean breeding method

A nematode and soybean resistance technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of slowing down the breeding process of cyst nematode disease resistance, destroying disease-resistant plants, and increasing the breeding workload, etc. The effect of overcoming the difficulty of breeding identification, realizing organic combination and improving breeding efficiency

Active Publication Date: 2014-01-15
CROP RES INST SHANDONG ACAD OF AGRI SCI
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Problems solved by technology

The identification of soybean cyst nematode resistance is generally carried out 35-40 days after sowing by using the method of investigating the number of cysts attached to the roots after rooting and washing. Continuous and stable inheritance in the breeding population brings difficulties to subsequent selection, resulting in low efficiency of soybean cyst nematode resistance breeding
At the same time, because soybean cyst nematode disease is a soil-borne disease, there are characteristics such as uneven distribution of soil pathogens and spot occurrence of disease, cyst nematode disease often occurs in spot patches in field disease nurseries, resulting in soybean hybrid offspring materials resistant to the disease Inaccurate sex identification, poor selection of disease-resistant plants, and low breeding efficiency
[0004] In addition, there is also a method of inducing rooting culture for the young part of soybean vegetative tissue to identify, but this method requires tissue culture and resistance identification of all breeding materials, which greatly increases the workload of breeding and slows down the resistance of cells. Cyst nematode breeding process, breeding efficiency is also low

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Embodiment Construction

[0020] The present invention will be further described below through specific examples. However, it should not be construed as limiting the protection scope of the claims of the present invention.

[0021] as attached figure 1 , 2 As shown, the specific process of each operation is:

[0022] Hybridization: select cyst nematode-resistant germplasm as parents to cross with main varieties with excellent comprehensive traits to build a cyst nematode-resistant breeding population;

[0023] Hybrid combination screening: screening dominant combinations in the F1 generation;

[0024] Identification of potted plants in low-generation diseased soil and selection of single nematode-resistant plants: identification of potted plants in F2 and F3 generation cyst nematode diseased soils, screening of single nematode-resistant plants according to the number of cysts in a single plant (the number of cysts in a single plant is less than 3), and transplanting The identification steps of pott...

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Abstract

The invention discloses an anti-nematode soybean breeding method. The anti-nematode soybean breeding method comprises the steps of hybridizing, screening hybridized combinations, performing low-generation sick soil potting identification, screening anti-nematode individual plants, comprehensively identifying advanced-generation field disease nurseries, screening good strains, comprehensively identifying and the like. The anti-nematode soybean breeding method can effectively overcome the technical problems that anti-cyst nematode soybean breeding identification is difficult, the identification is disjointed with selection, the selection is hard, and the breeding efficiency is low. The anti-nematode soybean breeding method realizes organic combination between root disease-resistant character identification and breeding, and improves anti-cyst nematode soybean breeding efficiency.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a breeding method for nematode-resistant soybeans. Background technique [0002] Soybean cyst nematode (SCN) is a devastating disease of soybean production, and it is also one of the main diseases of soybean planting in my country. The Huanghuaihai and Northeast soybean production areas have suffered more serious damage, and severe plots have lost more than 50% of their production, or even ceased production. Breeding and promoting nematode-resistant soybean varieties is the most cost-effective control measure. [0003] At present, the selection of hybrid offspring in soybean breeding is the selection of shoot traits in the late growth period, while the selection of root traits is a major problem in soybean breeding. Soybean cyst nematode is a root disease, and there are problems such as difficult identification of resistance, difficult selection, and low breeding efficiency. Soybe...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 徐冉王彩洁张礼凤李伟张彦威
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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