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Paper-based electrospray ionization mass spectrometry fingerprint technology and construction method thereof

A technology of mass spectrometry fingerprinting and electrospray mass spectrometry, which is applied in the field of fingerprinting technology and its construction, can solve the problems of chromatographic separation that takes a long time and cannot meet the needs of fast and high-throughput analysis, and achieve high-throughput analysis, The effect of accurate authenticity

Inactive Publication Date: 2014-01-08
CHINA NAT ANALYTICAL CENT GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for traditional Chinese medicines or traditional Chinese medicine preparations with complex systems, high performance liquid chromatography requires a long time for chromatographic separation, and complex and tedious sample pretreatment is required before instrumental analysis, which makes this technology unable to meet the requirements of fast, high-efficiency Throughput Analysis Requirements

Method used

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  • Paper-based electrospray ionization mass spectrometry fingerprint technology and construction method thereof
  • Paper-based electrospray ionization mass spectrometry fingerprint technology and construction method thereof
  • Paper-based electrospray ionization mass spectrometry fingerprint technology and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Establishment of the paper-based electrospray mass spectrometry fingerprint test method for the herbal tea of ​​Dasha

[0022] 1. Instruments and reagents

[0023] 1.1 Instruments Agilent Trap XCT ion trap mass spectrometer, LCMSD Trap5.2 mass spectrometry workstation.

[0024] 1.2 Reagents Chromatographic paper G4, methanol (chromatographically pure), twice redistilled water.

[0025] 2. Experimental part

[0026] 2.1 Preparation of the test solution

[0027] Pasha herbal tea was directly used for paper-based electrospray mass spectrometry analysis without sample pretreatment.

[0028] 2.2 Paper-based electrospray mass spectrometry test

[0029] Use chromatographic paper to cut G4 to make an isosceles triangular paper-based carrier. The size of the paper-based carrier is about 5 mm wide at the base and about 10 mm high. The paper-based carrier was fixed on the three-dimensional mobile stage with stainless steel tweezers, so that the tip of the top corne...

Embodiment 2

[0032] Example 2. The standard fingerprints of Huangzhenlong Pasha Herbal Tea and Pingantang Pasha Herbal Tea

[0033] 18 batches of qualified Huangzhenlong Pasha herbal tea samples and 18 batches of qualified Pingantang Pasha herbal tea samples were taken, and the paper-based electrospray mass spectrometry fingerprint analysis test was carried out according to the conditions of Example 1. To avoid background signal interference from paper-based carriers and spray solvents, these background signals will be subtracted from the fingerprints. Each batch of samples was tested in parallel three times, and the average mass spectrum obtained after deducting the background signal was output as a .txt file as the fingerprint of the sample. The average method was used to calculate and generate the standard fingerprints of the two sources of Sha Sha herbal tea, see the attached figure 2 (a-b). The characteristics of the standard fingerprints of Huang Zhenlong's Sha Sha herbal tea were...

Embodiment 3

[0035] Example 3. Paper-based electrospray mass spectrometry fingerprints for quality evaluation and source identification of Zha Sha herbal tea

[0036] Take 18 batches of qualified Huangzhenlong Xie Sha herbal tea, 18 batches of qualified Pingantang Xie Sha herbal tea, 3 batches of expired Huangzhenlong Xie Sha herbal tea, and 3 batches of expired Pingan Tang Xie Sha herbal tea, and carry out the paper according to the conditions of Example 1. Based on the electrospray mass spectrometry fingerprint analysis test, each sample was paralleled three times, and the average mass spectrum obtained from the three experiments was used as the fingerprint of the sample after deducting the background, and was output as a .csv file. Statistical software was used to conduct principal component analysis (PCA) on the paper-based electrospray mass spectrometry fingerprint of Husha herbal tea. The PCA score map and loading map are shown in the appendix image 3 . The 12 characteristic ions t...

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Abstract

The invention provides a paper-based electrospray ionization mass spectrometry fingerprint technology and a construction method thereof. The paper-based electrospray ionization mass spectrometry fingerprint technolog comprises the steps of (1) manufacturing a paper-based carrier; (2) performing a paper-based eelctrospray test: aligning the tip end of the paper-based carrier to a mass spectrometry entrance, dropping a sample at the center position, loading a high-voltage electric field, dropping a spray solvent for dissolving active components in the sample, moving to the tip end of the paper-based carrier under the action of the high-voltage electric field to form a Taylor cone at the tip end, generating charged spray liquid drops, ionizing the active components and performing the mass spectrometry; (3) recording the mass spectrometry; (4) obtaining a fingerprint; (5) obtaining a standard fingerprint: calculating the standard fingerprint by an averaging method; (6) analyzing main components: performing quality evaluation, true and false identification and source distinguishing through main component analysis. The invention provides a brand-new high-flux mass spectrometry fingerprint analysis method which does not comprise sample pretreatment and chromatographic separation, thus quality monitoring for liquid Chinese herbal preparations or an extracting solution sample is facilitated.

Description

technical field [0001] The present invention relates to a brand-new fingerprint technology and its construction method, in particular to the use of paper-based electrospray ionization technology to directly construct mass spectrum fingerprints based on active components of liquid Chinese medicine preparations or extract samples, so as to grasp the liquid Chinese medicine from the overall appearance Intrinsic quality of preparation or extract. Background technique [0002] Fingerprint is an effective means to characterize the relationship between the components of traditional Chinese medicine or traditional Chinese medicine preparations and their quality, and is an effective means to evaluate the overall quality of traditional Chinese medicine or traditional Chinese medicine preparations. China and the State Food and Drug Administration of the United States, the German Society of Medicinal Botany, the British Herbal Pharmacopoeia, the Indian Herbal Pharmacopoeia, and the Cana...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
Inventor 杨运云邓洁薇宋化灿
Owner CHINA NAT ANALYTICAL CENT GUANGZHOU
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