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A kind of method of producing giant salamander polypeptide powder

A giant salamander polypeptide and giant salamander technology, applied in the field of biomedicine, can solve problems such as unclear functions, and achieve the effect of anti-oxidative activity, physical fatigue, high safety, and alleviating physical fatigue

Inactive Publication Date: 2015-12-02
四川龙王洞生态农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, there are also studies on the hydrolysis of giant salamander proteases. The preparation of the few giant salamander polypeptides is limited to the optimization of enzymatic hydrolysis conditions, and its functions are limited to methods such as relieving physical fatigue; Chinese patent publication "CN102676622A" discloses "Great salamander nanopeptides and giant salamander wine" It can be seen from the patent application that the method is only to obtain giant salamander "nano-peptide" and more free amino acids by obtaining nano-sized giant salamander powder through simple enzymatic hydrolysis. The resulting product is questionable, and its function is not very clear.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 1000 grams of cut fresh giant salamander meat, dry it at 60°C to get dried giant salamander meat (moisture content is 3-5%), grind it through 60 mesh, and get giant salamander meat powder, which can be used for edible oil extraction with 6# solvent Oil treatment removes fat. The solvent was evaporated to obtain defatted giant salamander powder. Take 0.1g of compound enzymes of animal hydrolytic protease and neutral protease (2:1), pretreat with 5g of 50℃ hot water for 10 minutes, stir well and filter, add to 100g of defatted giant salamander powder and 300g of purified water In the system, no unsafe buffer solution was used to adjust the pH value, and the enzymatic hydrolysis was controlled for 1.5 hours at natural pH and 45°C. Then the enzymatic solution is centrifuged, filtered, and the filtrate is taken, and then filtered through an ultra-membrane twice, the first time is filtered with a membrane with a molecular weight cut-off of 2000 Daltons, and the retentat...

Embodiment 2

[0021] Take 2,000 grams of fresh giant salamander meat, dry it at 65°C to get dried giant salamander meat (moisture content is 3-5%), crush it through 80 meshes to get giant salamander meat powder, and remove it by treating with 6# solvent oil that can be used for edible oil extraction Fat. The solvent was evaporated to obtain defatted giant salamander powder. Take 0.2 grams of compound enzymes of animal hydrolytic protease and neutral protease (2:1) with 100,000 activity units each, pretreat with 5 grams of 50°C hot water for 10 minutes, stir well and filter, add to 200 grams of defatted giant salamander powder and pure In the system of 600 grams of water, no unsafe buffer solution was used to adjust the pH value, and the enzymatic hydrolysis was controlled for 1 hour at natural pH and 50°C. Then centrifuge the enzymatic solution, filter it, take the filtrate, and then pass it through ultra-membrane filtration twice. After passing through the liquid, a giant salamander poly...

Embodiment 3

[0023] Take 2,000 grams of fresh giant salamander meat, dry it at 65°C to obtain dried giant salamander meat (moisture content is 3-5%), grind it through 40 meshes, and obtain giant salamander meat powder, which can be removed by 6# solvent oil that can be used for edible oil extraction Fat. The solvent was evaporated to obtain defatted giant salamander powder. Take 0.2 grams of compound enzymes of animal hydrolytic protease and neutral protease (2:1) with 100,000 activity units each, pretreat with 5 grams of 50°C hot water for 10 minutes, stir well and filter, add to 200 grams of defatted giant salamander powder and pure In the system of 600 grams of water, no unsafe buffer solution was used to adjust the pH value, and the enzymatic hydrolysis was controlled for 50 minutes at natural pH and 48°C. Then centrifuge the enzymatic solution, filter it, take the filtrate, and then pass it through ultra-membrane filtration twice. After passing through the liquid, a giant salamander...

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Abstract

The invention discloses a method for producing giant salamander polypeptide powder, which comprises the following steps of low-temperature drying, crushing, fat removing, controlled enzymatic hydrolysis with a compound enzyme, centrifugation, coarse filtration, two times of membrane filtration, vacuum concentration, freeze drying, and pulverization of fresh giant salamander meat to obtain the giant salamander polypeptide powder.

Description

technical field [0001] The invention relates to a method for producing giant salamander polypeptide, which belongs to the field of biomedicine. Background technique [0002] As the only existing large amphibian, giant salamander is being deeply recognized and studied by people. At present, people's research on giant salamander mainly focuses on ecological investigation, morphological anatomy, artificial breeding and breeding. With the success of artificial breeding, the importance of giant salamander academic research has been further sublimated, and people's understanding of the edible value and efficacy of giant salamander has continued to deepen. Unfortunately, there are very few deep-processed products that can be called giant salamanders. Some so-called giant salamander peptide products on the market are just giant salamander protein. Because peptides have more and stronger activity and diversity than other substances in organisms (including proteins and amino acids),...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/34A23J3/04A23J1/02C12P21/06A61K38/01A61P1/16A61P37/02A61P39/06A61P39/00
Inventor 刘绍阳爱生邱平
Owner 四川龙王洞生态农业开发有限公司
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