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Method for immobilization of lipase and its application

A technology of immobilizing lipase and fat, applied to the direction of immobilizing on or in inorganic carriers, chemical instruments and methods, silicon dioxide, etc., can solve the problems of increasing costs, waste of raw materials, waste of raw materials, etc.

Active Publication Date: 2013-12-25
FENGHAI PANJIN RICE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, the existing TL-immobilized enzymes on the market cannot meet the production requirements
A large amount of soap is found in the product in the catalyzed transesterification reaction, which wastes a lot of raw materials and increases the cost
Therefore, the current immobilized TL requires a large amount of oil to be eluted, resulting in a large amount of waste of raw materials
At the same time, it brings great difficulties to the downstream separation and purification, which limits its application in the oil industry.

Method used

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  • Method for immobilization of lipase and its application
  • Method for immobilization of lipase and its application
  • Method for immobilization of lipase and its application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0112] The raw materials for the preparation of white carbon black and activated carbon are rice husk ash. Not specifically limited, the white carbon black and activated carbon in the examples can also come from other sources, such as commercially available.

[0113] The preparation of rice husk ash in this example: put the rice husk into the boiler, control the combustion temperature at 600-700°C, and make the rice husk be heated evenly during the combustion process, and control the combustion time within 20-60 minutes, so that the carbon content Stable between 10-30%, the organic impurities contained in the rice husk are removed with the boiler induced draft system, and the rice husk ash discharged from the bottom of the fire grate is the raw material when the carbon content is in the range of 15%-25%. The rice husk ash is sieved through a 1-5 mm linear sieve to remove impurities such as brown rice stones to obtain the raw material of rice husk ash for preparing white carbon...

Embodiment 1

[0119] Embodiment 1: prepare white carbon black and activated carbon from rice husk ash

[0120] 1. Preparation of activated carbon 1: Weigh 300g of rice husk ash raw material, 96g of sodium hydroxide, add to the autoclave, then add 900mL of water, the reaction pressure is controlled at 4kg, the reaction time is 4h, filter and wash to obtain the filtrate and filter cake , the obtained filtrate is water glass, and the modulus of water glass filtrate is 2.5. Add one-fold amount of water to the obtained filter cake and mix, control the carbon content at about 15%, stir and heat to 50°C, and flow into it at a flow rate of 10m 3 Add dilute sulfuric acid with a concentration of 10% at a speed of / h, adjust the pH of the system to 3.5 within 10-30 minutes, filter and wash, and obtain activated carbon 1 after drying.

[0121]Preparation of white carbon black 1: first add 300g of water glass filtrate obtained by filtration into the reactor with a modulus of 2.5 and a specific gravity o...

Embodiment 2

[0129] Embodiment 2: the detection embodiment of white carbon black and activated carbon

[0130] The gac prepared in Example 1 and white carbon black are detected by national detection method GB / T12496-1999 and HG / T3061-1999, and the results are as follows:

[0131] Table 1 Detection index of activated carbon

[0132]

[0133] Note: BET is specific surface area, unit m 2 / g.

[0134] It can be shown from the detection data that the activated carbon prepared by the method of the present invention also contains silicon dioxide, and the obtained product is a mixture of activated carbon and silicon dioxide.

[0135] Table 2 Detection index of white carbon black

[0136] example

[0137] Note: BET is specific surface area, unit m 2 / g, DBP is the oil absorption value, and the unit is weight%.

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Abstract

The invention relates to a method for immobilization of lipase, and specifically relates to a method for immobilization of lipase by a white carbon black carrier. The steps include: providing awhite carbon black carrier, enabling white carbon black to contact and a lipase liquid, and making the lipase immobilized on the carrier; and then drying the immobilized lipase. The obtained immobilized lipase had good ester exchange activity, and after ester exchange, oil has a low soap content.

Description

technical field [0001] The invention relates to a method for immobilizing lipase and its application, in particular to a method for immobilizing lipase with white carbon black and its application. Background technique [0002] As a kind of enzyme that catalyzes oil hydrolysis, transesterification and esterification, lipase is widely used in food processing, medicine, papermaking, fine chemical industry and other industries because of its mild catalytic conditions and high selectivity. However, free enzymes are difficult to achieve large-scale industrial production because of their poor stability and difficulty in separating them from the product. Immobilized enzyme technology is a technology that binds free enzymes or confines them in a certain area by various means, so that they can still perform unique catalytic reactions and can be recycled and reused. Due to the above advantages, many scientific researches at home and abroad Workers have conducted extensive research on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C01B33/12C01B31/08C01B32/324
Inventor 穆彦魁郑妍辛本荣杨天奎徐学兵李超张贵银
Owner FENGHAI PANJIN RICE BIOTECH CO LTD
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